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PMID: 9548982 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Uncoupling PR gene expression from NPR1 and bacterial resistance: characterization of the dominant Arabidopsis cpr6-1 mutant.

The Plant cell ·Vol. 10 ·No. 4 ·1998-04-00 ·Pages 557-69

Clarke JD, Liu Y, Klessig DF, Dong X

Abstract

In Arabidopsis, NPR1 mediates the salicylic acid (SA)-induced expression of pathogenesis-related (PR) genes and systemic acquired resistance (SAR). Here, we report the identification of another component, CPR 6, that may function with NPR1 in regulating PR gene expression. The dominant CPR 6-1 mutant expresses the SA/NPR1-regulated PR genes (PR-1, BGL 2, and PR-5) and displays enhanced resistance to Pseudomonas syringae pv maculicola ES4326 and Peronospora parasitica Noco2 in the absence of SAR induction. cpr 6-1-induced PR gene expression is not suppressed in the cpr 6-1 npr1-1 double mutant but is suppressed when SA is removed by salicylate hydroxylase. Thus, constitutive PR gene expression in cpr 6-1 requires SA but not NPR1. In addition, resistance to P. s. maculicola ES4326 is suppressed in the cpr 6-1 npr1-1 double mutant, despite expression of PR-1, BGL 2, and PR-5. Resistance to P. s. maculicola ES4326 must therefore be accomplished through unidentified antibacterial gene products that are regulated through NPR1. These results show that CPR 6 is an important regulator of multiple signal transduction pathways involved in plant defense.

MeSH Terms
Arabidopsis/genetics,microbiology,physiology Arabidopsis Proteins Carrier Proteins/genetics Cyclophilin D Cyclophilins Epistasis, Genetic Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Genes, Dominant Genes, Plant Mutation Oomycetes/pathogenicity Peptidylprolyl Isomerase/genetics Plant Diseases/genetics,microbiology Plant Proteins/genetics Pseudomonas/pathogenicity Salicylates/metabolism Salicylic Acid
Chemicals
Arabidopsis Proteins Carrier Proteins Cyclophilin D NPR1 protein, Arabidopsis Plant Proteins Salicylates pathogenesis-related proteins, plant Cyclophilins Peptidylprolyl Isomerase Salicylic Acid
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Clarke J D
Developmental, Cell, and Molecular Biology Group, Department of Botany, Box 91000, Duke University, Durham, North Carolina 27708-1000, USA.
Liu Y
Klessig D F
Dong X
References (55)
55 references, click to expand
  1. Suppression and Restoration of Lesion Formation in Arabidopsis lsd Mutants.
    Plant Cell. 1995 Dec;7(12):2013-2022 PMID: 12242366
  2. Thionins: properties, possible biological roles and mechanisms of action.
    Plant Mol Biol. 1994 Oct;26(1):25-37 PMID: 7948874
  3. Characterization of a salicylic acid-insensitive mutant (sai1) of Arabidopsis thaliana, identified in a selective screen utilizing the SA-inducible expression of the tms2 gene.
    Mol Plant Microbe Interact. 1997 Jan;10(1):69-78 PMID: 9002272
  4. Polyacrylamide disc electrophoresis of the soluble leaf proteins from Nicotiana tabacum var. "Samsun" and "Samsun NN". II. Changes in protein constitution after infection with tobacco mosaic virus.
    Virology. 1970 Feb;40(2):190-211 PMID: 4191688
  5. An Arabidopsis thaliana thionin gene is inducible via a signal transduction pathway different from that for pathogenesis-related proteins.
    Plant Physiol. 1995 Nov;109(3):813-20 PMID: 8552715
  6. Arabidopsis enhanced disease susceptibility mutants exhibit enhanced susceptibility to several bacterial pathogens and alterations in PR-1 gene expression.
    Plant Cell. 1997 Mar;9(3):305-16 PMID: 9090877
  7. The Arabidopsis NIM1 protein shows homology to the mammalian transcription factor inhibitor I kappa B.
    Plant Cell. 1997 Mar;9(3):425-39 PMID: 9090885
  8. Pathogen-induced proteins with inhibitory activity toward Phytophthora infestans.
    Plant Cell. 1991 Jun;3(6):619-28 PMID: 1841721
  9. Genomic sequencing.
    Proc Natl Acad Sci U S A. 1984 Apr;81(7):1991-5 PMID: 6326095
  10. Nucleotide sequence analysis of the Pseudomonas putida PpG7 salicylate hydroxylase gene (nahG) and its 3'-flanking region.
    Biochemistry. 1991 Feb 12;30(6):1635-41 PMID: 1993181
  11. Interference between Two Specific Pathogen Recognition Events Mediated by Distinct Plant Disease Resistance Genes.
    Plant Cell. 1996 Feb;8(2):251-257 PMID: 12239384
  12. Cloning and expression of a PR5-like protein from Arabidopsis: inhibition of fungal growth by bacterially expressed protein.
    Plant Mol Biol. 1997 Aug;34(6):949-59 PMID: 9290646
  13. Signal molecules in systemic plant resistance to pathogens and pests.
    Cell. 1992 Sep 18;70(6):879-86 PMID: 1525827
  14. Biological function of ;pathogenesis-related' proteins: four PR proteins of tobacco have 1,3-beta-glucanase activity.
    EMBO J. 1987 Nov;6(11):3209-12 PMID: 16453802
  15. A mutation in Arabidopsis that leads to constitutive expression of systemic acquired resistance.
    Plant Cell. 1994 Dec;6(12):1845-57 PMID: 7866028
  16. Small cysteine-rich antifungal proteins from radish: their role in host defense.
    Plant Cell. 1995 May;7(5):573-88 PMID: 7780308
  17. Arabidopsis mutants simulating disease resistance response.
    Cell. 1994 May 20;77(4):565-77 PMID: 8187176
  18. Initiation of runaway cell death in an Arabidopsis mutant by extracellular superoxide.
    Science. 1996 Sep 27;273(5283):1853-6 PMID: 8791589
  19. Arabidopsis signal transduction mutant defective in chemically and biologically induced disease resistance.
    Proc Natl Acad Sci U S A. 1995 Jul 3;92(14):6602-6 PMID: 11607555
  20. Pathogen-induced systemic activation of a plant defensin gene in Arabidopsis follows a salicylic acid-independent pathway.
    Plant Cell. 1996 Dec;8(12):2309-23 PMID: 8989885
  21. Salicylic Acid: a likely endogenous signal in the resistance response of tobacco to viral infection.
    Science. 1990 Nov 16;250(4983):1002-4 PMID: 17746925
  22. Increase in salicylic Acid at the onset of systemic acquired resistance in cucumber.
    Science. 1990 Nov 16;250(4983):1004-6 PMID: 17746926
  23. The generation of radiolabeled DNA and RNA probes with polymerase chain reaction.
    Anal Biochem. 1989 Feb 15;177(1):90-4 PMID: 2742155
  24. Salicylic Acid Inhibits Synthesis of Proteinase Inhibitors in Tomato Leaves Induced by Systemin and Jasmonic Acid.
    Plant Physiol. 1995 Aug;108(4):1741-1746 PMID: 12228577
  25. Molecular genetics of plant disease resistance.
    Science. 1995 May 5;268(5211):661-7 PMID: 7732374
  26. The cpr5 mutant of Arabidopsis expresses both NPR1-dependent and NPR1-independent resistance.
    Plant Cell. 1997 Sep;9(9):1573-84 PMID: 9338960
  27. Isolation of Arabidopsis genes that differentiate between resistance responses mediated by the RPS2 and RPM1 disease resistance genes.
    Plant Cell. 1996 Feb;8(2):241-9 PMID: 8742710
  28. Benzothiadiazole, a novel class of inducers of systemic acquired resistance, activates gene expression and disease resistance in wheat.
    Plant Cell. 1996 Apr;8(4):629-43 PMID: 8624439
  29. Assignment of 30 microsatellite loci to the linkage map of Arabidopsis.
    Genomics. 1994 Jan 1;19(1):137-44 PMID: 8188214
  30. Resistance gene-dependent plant defense responses.
    Plant Cell. 1996 Oct;8(10):1773-91 PMID: 8914325
  31. Signaling in plant-microbe interactions.
    Science. 1997 May 2;276(5313):726-33 PMID: 9115193
  32. Benzothiadiazole induces disease resistance in Arabidopsis by activation of the systemic acquired resistance signal transduction pathway.
    Plant J. 1996 Jul;10(1):71-82 PMID: 8758979
  33. Programmed cell death in plants: a pathogen-triggered response activated coordinately with multiple defense functions.
    Cell. 1994 May 20;77(4):551-63 PMID: 8187175
  34. Overexpression of an endogenous thionin enhances resistance of Arabidopsis against Fusarium oxysporum.
    Plant Cell. 1997 Apr;9(4):509-20 PMID: 9144959
  35. Systemic acquired resistance induced by localized virus infections in plants.
    Virology. 1961 Jul;14:340-58 PMID: 13743578
  36. Requirement of salicylic Acid for the induction of systemic acquired resistance.
    Science. 1993 Aug 6;261(5122):754-6 PMID: 17757215
  37. GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.
    EMBO J. 1987 Dec 20;6(13):3901-7 PMID: 3327686
  38. The Arabidopsis NPR1 gene that controls systemic acquired resistance encodes a novel protein containing ankyrin repeats.
    Cell. 1997 Jan 10;88(1):57-63 PMID: 9019406
  39. Coordinate Gene Activity in Response to Agents That Induce Systemic Acquired Resistance.
    Plant Cell. 1991 Oct;3(10):1085-1094 PMID: 12324583
  40. Isolation of Arabidopsis mutants with enhanced disease susceptibility by direct screening.
    Genetics. 1996 Jun;143(2):973-82 PMID: 8725243
  41. A procedure for mapping Arabidopsis mutations using co-dominant ecotype-specific PCR-based markers.
    Plant J. 1993 Aug;4(2):403-10 PMID: 8106085
  42. Systemic Induction of Salicylic Acid Accumulation in Cucumber after Inoculation with Pseudomonas syringae pv syringae.
    Plant Physiol. 1991 Dec;97(4):1342-7 PMID: 16668554
  43. Plant Disease Resistance Genes: Function Meets Structure.
    Plant Cell. 1996 Oct;8(10):1757-1771 PMID: 12239361
  44. Acquired resistance in Arabidopsis.
    Plant Cell. 1992 Jun;4(6):645-56 PMID: 1392589
  45. Salicylate-independent lesion formation in Arabidopsis lsd mutants.
    Mol Plant Microbe Interact. 1997 Jul;10(5):531-6 PMID: 9204559
  46. Pathogenesis-related PR-1 proteins are antifungal. Isolation and characterization of three 14-kilodalton proteins of tomato and of a basic PR-1 of tobacco with inhibitory activity against Phytophthora infestans.
    Plant Physiol. 1995 May;108(1):17-27 PMID: 7784503
  47. Gene-Expression Patterns and Levels of Jasmonic Acid in Rice Treated with the Resistance Inducer 2,6-Dichloroisonicotinic Acid.
    Plant Physiol. 1997 Sep;115(1):61-70 PMID: 12223792
  48. Systemic Acquired Resistance.
    Plant Physiol. 1994 Apr;104(4):1109-1112 PMID: 12232151
  49. Salicylic acid is a systemic signal and an inducer of pathogenesis-related proteins in virus-infected tobacco.
    Plant Cell. 1991 Aug;3(8):809-18 PMID: 1820820
  50. Temperature-Dependent Induction of Salicylic Acid and Its Conjugates during the Resistance Response to Tobacco Mosaic Virus Infection.
    Plant Cell. 1992 Mar;4(3):359-366 PMID: 12297650
  51. A central role of salicylic Acid in plant disease resistance.
    Science. 1994 Nov 18;266(5188):1247-50 PMID: 17810266
  52. Characterization of an Arabidopsis Mutant That Is Nonresponsive to Inducers of Systemic Acquired Resistance.
    Plant Cell. 1994 Nov;6(11):1583-1592 PMID: 12244227
  53. Systemic resistance in Arabidopsis induced by biocontrol bacteria is independent of salicylic acid accumulation and pathogenesis-related gene expression.
    Plant Cell. 1996 Aug;8(8):1225-37 PMID: 8776893
  54. Systemic Acquired Resistance.
    Plant Cell. 1996 Oct;8(10):1809-1819 PMID: 12239363
  55. Plant defensins: novel antimicrobial peptides as components of the host defense system.
    Plant Physiol. 1995 Aug;108(4):1353-8 PMID: 7659744
Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.10.4.557
Published
1998-04-00
Pages
557-69
Language
English
Region
England
NLM ID
9208688
PMCID
PMC144011
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.10.4.557 PMC Full Text View journal details
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