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PMID: 15994909 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

RAC GTPases in tobacco and Arabidopsis mediate auxin-induced formation of proteolytically active nuclear protein bodies that contain AUX/IAA proteins.

The Plant cell ·Vol. 17 ·No. 8 ·2005-08-00 ·Pages 2369-83

Tao LZ, Cheung AY, Nibau C, Wu HM

Abstract

Auxin signaling relies on ubiquitin ligase SCF(TIR1)-mediated 26S proteasome-dependent proteolysis of a large family of short-lived transcription regulators, auxin/indole acetic acid (Aux/IAA), resulting in the derepression of auxin-responsive genes. We have shown previously that a subset of Rac GTPases is activated by auxin, and they in turn stimulate auxin-responsive gene expression. We show here that increasing Rac signaling activity promotes Aux/IAA degradation, whereas downregulating that activity results in the reduction of auxin-accelerated Aux/IAA proteolysis. Observations reported here reveal a novel function for these Rac GTPases as regulators for ubiquitin/26S proteasome-mediated proteolysis and further consolidate their role in auxin signaling. Moreover, our study reveals a cellular process whereby auxin induces and Rac GTPases mediate the recruitment of nucleoplasmic Aux/IAAs into proteolytically active nuclear protein bodies, into which components of the SCF(TIR1), COP9 signalosome, and 26S proteasome are also recruited.

MeSH Terms
Arabidopsis Proteins/metabolism Genes, Reporter Indoleacetic Acids/metabolism Iodoacetates/metabolism Kinetics Nuclear Proteins/metabolism Plant Proteins/metabolism rac GTP-Binding Proteins/metabolism
Chemicals
Arabidopsis Proteins Indoleacetic Acids Iodoacetates Nuclear Proteins Plant Proteins rac GTP-Binding Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Tao Li-Zhen
Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst, Massachusetts 01003, USA.
Cheung Alice Y
Nibau Candida
Wu Hen-Ming
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.105.032987
Published
2005-08-00
Epub
2005-00-01
Pages
2369-83
Language
English
Region
England
NLM ID
9208688
PMCID
PMC1182495
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.105.032987 PMC Full Text View journal details
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