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PMID: 16006579 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The Putative RNA-dependent RNA polymerase RDR6 acts synergistically with ASYMMETRIC LEAVES1 and 2 to repress BREVIPEDICELLUS and MicroRNA165/166 in Arabidopsis leaf development.

The Plant cell ·Vol. 17 ·No. 8 ·2005-08-00 ·Pages 2157-71

Li H, Xu L, Wang H, Yuan Z, Cao X, Yang Z, Zhang D, Xu Y, Huang H

Abstract

The Arabidopsis thaliana ASYMMETRIC LEAVES1 (AS1) and AS2 genes are important for repressing class I KNOTTED1-like homeobox (KNOX) genes and specifying leaf adaxial identity in leaf development. RNA-dependent RNA polymerases (RdRPs) are critical for posttranscriptional and transcriptional gene silencing in eukaryotes; however, very little is known about their functions in plant development. Here, we show that the Arabidopsis RDR6 gene (also called SDE1 and SGS2) that encodes a putative RdRP, together with AS1 and AS2, regulates leaf development. rdr6 single mutant plants displayed only minor phenotypes, whereas rdr6 as1 and rdr6 as2 double mutants showed dramatically enhanced as1 and as2 phenotypes, with severe defects in the leaf adaxial-abaxial polarity and vascular development. In addition, the double mutant plants produced more lobed leaves than the as1 and as2 single mutants and showed leaf-like structures associated on a proportion of leaf blades. The abnormal leaf morphology of the double mutants was accompanied by an extended ectopic expression of a class I KNOX gene BREVIPEDICELLUS (BP) and high levels of microRNA165/166 that may lead to mRNA degradation of genes in the class III HD-ZIP family. Taken together, our data suggest that the Arabidopsis RDR6-associated epigenetic pathway and the AS1-AS2 pathway synergistically repress BP and MIR165/166 for proper plant development.

MeSH Terms
Arabidopsis/genetics Arabidopsis Proteins/metabolism Base Sequence Cell Polarity Phenotype Plant Leaves/genetics,growth & development,ultrastructure Plants, Genetically Modified/genetics RNA-Dependent RNA Polymerase/metabolism Reverse Transcriptase Polymerase Chain Reaction Transcription Factors/metabolism
Chemicals
AS2 protein, Arabidopsis ASYMMETRIC LEAVES1 protein, Arabidopsis Arabidopsis Proteins Transcription Factors RNA-Dependent RNA Polymerase
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Li Hong
National Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Shanghai Institute for Biological Sciences, Graduate School of Chinese Academy of Sciences, Shanghai 200032, China.
Xu Lin
Wang Hua
Yuan Zheng
Cao Xiaofeng
Yang Zhongnan
Zhang Dabing
Xu Yuquan
Huang Hai
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.105.033449
Published
2005-08-00
Epub
2005-00-08
Pages
2157-71
Language
English
Region
England
NLM ID
9208688
PMCID
PMC1182480
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.105.033449 PMC Full Text View journal details
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