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PMID: 1600939 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The yeast WBP1 is essential for oligosaccharyl transferase activity in vivo and in vitro.

The EMBO journal ·Vol. 11 ·No. 6 ·1992-06-00 ·Pages 2071-5

te Heesen S, Janetzky B, Lehle L, Aebi M

Abstract

Asparagine-linked N-glycosylation is a highly conserved and functionally important modification of proteins in eukaryotic cells. The central step in this process is a cotranslational transfer of lipid-linked core oligosaccharides to selected Asn-X-Ser/Thr-sequences of nascent polypeptide chains, catalysed by the enzyme N-oligosaccharyl transferase. In this report we show that the essential yeast protein WBP1 (te Heesen et al., 1991) is required for N-oligosaccharyl transferase in vivo and in vitro. Depletion of WBP1 correlates with a defect in transferring core oligosaccharides to carboxypeptidase Y and proteinase A in vivo. In addition, in vitro N-glycosylation of the acceptor peptide Tyr-Asn-Leu-Thr-Ser-Val using microsomal membranes from WBP1 depleted cells is reduced as compared with membranes from wild-type cells. We propose that WBP1 is an essential component of the oligosaccharyl transferase in yeast.

MeSH Terms
Alleles Amino Acid Sequence Aspartic Acid Endopeptidases/metabolism Base Sequence Carboxypeptidases/metabolism Cathepsin A Fungal Proteins/genetics,metabolism Galactosidases/genetics,metabolism Genes, Fungal Glycosylation Hexosyltransferases Membrane Proteins Microsomes/enzymology Molecular Sequence Data Oligodeoxyribonucleotides Plasmids Promoter Regions, Genetic Recombinant Fusion Proteins/metabolism Restriction Mapping Saccharomyces cerevisiae/enzymology,genetics,growth & development,metabolism Saccharomyces cerevisiae Proteins Substrate Specificity Temperature Transferases/genetics,metabolism
Chemicals
Fungal Proteins Membrane Proteins Oligodeoxyribonucleotides Recombinant Fusion Proteins Saccharomyces cerevisiae Proteins Transferases Hexosyltransferases dolichyl-diphosphooligosaccharide - protein glycotransferase Galactosidases Carboxypeptidases Cathepsin A PRC1 protein, S cerevisiae serine carboxypeptidase aspartic proteinase A Aspartic Acid Endopeptidases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
te Heesen S
Institut für Molekularbiologie I, Universität Zürich, Hönggerberg, Switzerland.
Janetzky B
Lehle L
Aebi M
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1992-06-00
Pages
2071-5
Language
English
Region
England
NLM ID
8208664
PMCID
PMC556673
Subset
IM
Databases
GENBANK
X61388, X64335, X64555, X64556, X64629, X64700, X67723, X67724, X67725, X67726
External Links
PubMed source PMC Full Text View journal details
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