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PMID: 16030216 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

An agr-like two-component regulatory system in Lactobacillus plantarum is involved in production of a novel cyclic peptide and regulation of adherence.

Journal of bacteriology ·Vol. 187 ·No. 15 ·2005-08-00 ·Pages 5224-35

Sturme MH, Nakayama J, Molenaar D, Murakami Y, Kunugi R, Fujii T, Vaughan EE, Kleerebezem M, de Vos WM

Abstract

We have analyzed a locus on the annotated Lactobacillus plantarum WCFS1 genome that showed homology to the staphylococcal agr quorum-sensing system and designated it lam for Lactobacillus agr-like module. Production of the lamBDCA transcript was shown to be growth phase dependent. Analysis of a response regulator-defective mutant (Delta)lamA) in an adherence assay showed that lam regulates adherence of L. plantarum to a glass surface. Global transcription analysis of the wild-type and (Delta)lamA strains in early, mid-, and late log phase of growth was performed using a clone-based microarray. Remarkably, only a small set of genes showed significant differences in transcription profiles between the wild-type and lamA mutant strains. The microarray analysis confirmed that lamBDCA is autoregulatory and showed that lamA is involved in regulation of expression of genes encoding surface polysaccharides, cell membrane proteins, and sugar utilization proteins. The lamBD genes encoding the putative autoinducing peptide precursor (LamD) and its processing protein (LamB) were overexpressed using the nisin-controlled expression system, and culture supernatants were analyzed by liquid chromatography/mass spectrometry (LC/MS) to identify overproduced LamD-derived peptides. In this way, a cyclic thiolactone pentapeptide that possesses a ring structure similar to those of autoinducing peptides of the staphylococcal agr system was identified. The peptide was designated LamD558, and its sequence (CVGIW) matched the annotated precursor peptide sequence. Time course analysis of wild-type culture supernatants by LC/MS indicated that LamD558 production was increased markedly from mid-log to late log growth phase. This is the first example of an agr-like system in nonpathogenic bacteria that encodes a cyclic thiolactone autoinducing peptide and is involved in regulation of adherence.

MeSH Terms
Amino Acid Sequence Bacterial Adhesion Bacterial Proteins/genetics,metabolism Genome, Bacterial Lactobacillus plantarum/growth & development,physiology Lipopolysaccharides/metabolism Molecular Sequence Data Operon/genetics Peptides, Cyclic/genetics,metabolism Sequence Alignment
Chemicals
Bacterial Proteins Lipopolysaccharides Peptides, Cyclic lipoarabinomannan
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Sturme Mark H J
Laboratory of Microbiology, Wageningen University, Hesselink van Suchtelenweg 4, 6703 CT Wageningen, The Netherlands. [email protected]
Nakayama Jiro
Molenaar Douwe
Murakami Yoshiko
Kunugi Ryoko
Fujii Toshio
Vaughan Elaine E
Kleerebezem Michiel
de Vos Willem M
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.187.15.5224-5235.2005
Email
[email protected]
Published
2005-08-00
Pages
5224-35
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC1196011
Subset
IM
External Links
PubMed source DOI: 10.1128/JB.187.15.5224-5235.2005 PMC Full Text View journal details
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