Cleavage of single-stranded DNA by the A and A* proteins of bacteriophage phi X174.

Nucleic acids research ·Vol. 7 ·No. 8 ·1979-12-20 ·Pages 2177-88

Langeveld SA, van Mansfeld AD, de Winter JM, Weisbeek PJ

Abstract

The purified A protein and A* protein of bacteriophage phi X174 have been tested for endonuclease activity on single stranded viral phi X174 DNA. The A protein (55.000 daltons) nicks single-stranded DNA in the same way and at the same place as it does superhelical RFI DNA, at the origin of DNA replication. The A* protein (37.000 daltons) can cleave the single-stranded viral DNA at many different sites. It has however a strong preference for the origin of replication. Both proteins generate 3'OH ends and blocked 5' termini at the nick site.

MeSH Terms

Bacteriophage phi X 174/enzymology Base Sequence DNA Nucleotidyltransferases/metabolism DNA, Single-Stranded DNA, Superhelical Deoxyribonucleases/metabolism Endonucleases/metabolism Molecular Weight Substrate Specificity Viral Proteins/isolation & purification,metabolism

Chemicals

DNA, Single-Stranded DNA, Superhelical Viral Proteins DNA Nucleotidyltransferases Deoxyribonucleases Endonucleases

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Langeveld S A

van Mansfeld A D

de Winter J M

Weisbeek P J

References (15)

15 references, click to expand

Isolation and characterization of the protein coded by gene A of bacteriophage phiX174 DNA.
Proc Natl Acad Sci U S A. 1976 Aug;73(8):2669-73 PMID: 1066678
Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis.
Biochemistry. 1975 Aug 26;14(17):3787-94 PMID: 1174504
Cleavage site of phiX174 gene-A protein in phiX and G4 RFI DNA.
Cold Spring Harb Symp Quant Biol. 1979;43 Pt 1:331-4 PMID: 157838
A new method for sequencing DNA.
Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
phiX174 cistron A protein is a multifunctional enzyme in DNA replication.
Proc Natl Acad Sci U S A. 1977 Aug;74(8):3198-202 PMID: 269383
Isolation and function of the gene A initiator of bacteriophage phi-chi 174, a highly specific DNA endonuclease.
Proc Natl Acad Sci U S A. 1974 Apr;71(4):1549-53 PMID: 4598307
DNA replication: the rolling circle model.
Cold Spring Harb Symp Quant Biol. 1968;33:473-84 PMID: 4891987
Formation of the parental replicative form DNA of bacteriophage phi-X174 and initial events in its replication.
J Mol Biol. 1971 Nov 14;61(3):565-86 PMID: 5133115
Nucleotide sequence of the origin of replication in bacteriophage phiX174 RF DNA.
Nature. 1978 Feb 2;271(5644):417-20 PMID: 628424
Nucleotide sequence of bacteriophage G4 DNA.
Nature. 1978 Nov 16;276(5685):236-47 PMID: 714153
The nucleotide sequence of bacteriophage phiX174.
J Mol Biol. 1978 Oct 25;125(2):225-46 PMID: 731693
Bacteriophage phiX174 DNA synthesis in a replication-deficient host: determination of the origin of phiX DNA replication.
J Mol Biol. 1976 Apr 15;102(3):633-56 PMID: 775113
'Single-stranded' DNA from phiX174 and M13 is cleaved by certain restriction endonucleases.
Nature. 1975 Oct 2;257(5525):421-2 PMID: 809717
Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid.
J Bacteriol. 1976 Sep;127(3):1529-37 PMID: 821935
Gene A product of phi X174 is required for site-specific endonucleolytic cleavage during single-stranded DNA synthesis in vivo.
J Virol. 1976 Aug;19(2):416-24 PMID: 957477

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/7.8.2177

Published

1979-12-20

Pages

2177-88

Language

English

Region

England

NLM ID

0411011

PMCID

PMC342378

Subset

External Links

PubMed source DOI: 10.1093/nar/7.8.2177 PMC Full Text View journal details

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Cleavage of single-stranded DNA by the A and A* proteins of bacteriophage phi X174.

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