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PMID: 16100512 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Protein kinase D regulates vesicular transport by phosphorylating and activating phosphatidylinositol-4 kinase IIIbeta at the Golgi complex.

Nature cell biology ·Vol. 7 ·No. 9 ·2005-09-00 ·Pages 880-6

Hausser A, Storz P, Märtens S, Link G, Toker A, Pfizenmaier K

Abstract

Protein kinase D (PKD) regulates the fission of vesicles originating from the trans-Golgi network. We show that phosphatidylinositol 4-kinase IIIbeta (PI4KIIIbeta) - a key player in the structure and function of the Golgi complex - is a physiological substrate of PKD. Of the three PKD isoforms, only PKD1 and PKD2 phosphorylated PI4KIIIbeta at a motif that is highly conserved from yeast to humans. PKD-mediated phosphorylation stimulated lipid kinase activity of PI4KIIIbeta and enhanced vesicular stomatitis virus G-protein transport to the plasma membrane. The identification of PI4KIIIbeta as one of the PKD substrates should help to reveal the molecular events that enable transport-carrier formation.

MeSH Terms
1-Phosphatidylinositol 4-Kinase/metabolism Amino Acid Motifs/physiology Animals Biological Transport, Active/physiology COS Cells Cell Membrane/enzymology Chlorocebus aethiops Conserved Sequence/physiology Enzyme Activation/physiology Evolution, Molecular Golgi Apparatus/enzymology,metabolism Humans Lipid Metabolism Membrane Glycoproteins/metabolism Phosphorylation Protein Isoforms/metabolism Protein Kinase C/metabolism Transport Vesicles/enzymology Viral Envelope Proteins/metabolism
Chemicals
G protein, vesicular stomatitis virus Membrane Glycoproteins Protein Isoforms Viral Envelope Proteins 1-Phosphatidylinositol 4-Kinase protein kinase D Protein Kinase C
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Hausser Angelika
Institute for Cell Biology and Immunology, University of Stuttgart, Allmandring 31, 70569 Stuttgart, Germany. [email protected]
Storz Peter
Märtens Susanne
Link Gisela
Toker Alex
Pfizenmaier Klaus
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26 references, click to expand
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Article Info
Journal
Nature cell biology
Abbr.
Nat Cell Biol
ISSN
1465-7392
DOI
10.1038/ncb1289
Email
[email protected]
Published
2005-09-00
Epub
2005-00-14
Pages
880-6
Language
English
Region
England
NLM ID
100890575
PMCID
PMC1458033
Subset
IM
Grants
NCI NIH HHS · R01 CA075134 · United States
NCI NIH HHS · CA075134 · United States
Corrections
CommentIn
PMID: 16136181
External Links
PubMed source DOI: 10.1038/ncb1289 PMC Full Text View journal details
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