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PMID: 16177898 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S.

BAC libraries of Triticum urartu, Aegilops speltoides and Ae. tauschii, the diploid ancestors of polyploid wheat.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik ·Vol. 111 ·No. 8 ·2005-11-00 ·Pages 1617-22

Akhunov ED, Akhunova AR, Dvorák J

Abstract

Triticum urartu, Aegilops speltoides and Ae. tauschii are respectively the immediate diploid sources, or their closest relatives, of the A, B and D genomes of polyploid wheats. Here we report the construction and characterization of arrayed large-insert libraries in a bacterial artificial chromosome (BAC) vector, one for each of these diploid species. The libraries are equivalent to 3.7, 5.4 and 4.1 of the T. urartu, Ae. speltoides, Ae. tauschii genomes, respectively. The predicted levels of genome coverage were confirmed by library hybridization with single-copy genes. The libraries were used to estimate the proportion of known repeated nucleotide sequences and gene content in each genome by BAC-end sequencing. Repeated sequence families previously detected in Triticeae accounted for 57, 61 and 57% of the T. urartu, Ae. speltoides and Ae. tauschii genomes, and coding regions accounted for 5.8, 4.5 and 4.8%, respectively.

MeSH Terms

Base Sequence Chromosomes, Artificial, Bacterial DNA Primers Genome, Plant/genetics Molecular Sequence Data Polyploidy Sequence Analysis, DNA Species Specificity Triticum/genetics

Chemicals

DNA Primers

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Akhunov E D

Department of Plant Sciences, University of California, Davis, CA 95616, USA.

Akhunova A R

Dvorák J

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21 references, click to expand

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Article Info

Journal

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik

Abbr.

Theor Appl Genet

ISSN

0040-5752

DOI

10.1007/s00122-005-0093-1

Published

2005-11-00

Epub

2005-00-10

Pages

1617-22

Language

English

Region

Germany

NLM ID

0145600

Subset

External Links

PubMed source DOI: 10.1007/s00122-005-0093-1 View journal details

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BAC libraries of Triticum urartu, Aegilops speltoides and Ae. tauschii, the diploid ancestors of polyploid wheat.

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