Home LiteratureArticle Details
PMID: 16204182 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

RNA Pol II subunit Rpb7 promotes centromeric transcription and RNAi-directed chromatin silencing.

Genes & development ·Vol. 19 ·No. 19 ·2005-10-01 ·Pages 2301-6

Djupedal I, Portoso M, Spåhr H, Bonilla C, Gustafsson CM, Allshire RC, Ekwall K

Abstract

Fission yeast centromeric repeats are transcribed into small interfering RNA (siRNA) precursors (pre-siRNAs), which are processed by Dicer to direct heterochromatin formation. Recently, Rpb1 and Rpb2 subunits of RNA polymerase II (RNA Pol II) were shown to mediate RNA interference (RNAi)-directed chromatin modification but did not affect pre-siRNA levels. Here we show that another Pol II subunit, Rpb7 has a specific role in pre-siRNA transcription. We define a centromeric pre-siRNA promoter from which initiation is exquisitely sensitive to the rpb7-G150D mutation. In contrast to other Pol II subunits, Rpb7 promotes pre-siRNA transcription required for RNAi-directed chromatin silencing.

MeSH Terms
Centromere/genetics,metabolism Chromatin/genetics,metabolism Gene Expression Regulation, Fungal/physiology Promoter Regions, Genetic/physiology RNA Interference/physiology RNA Polymerase II/genetics,metabolism RNA Processing, Post-Transcriptional/physiology RNA, Small Interfering/genetics,metabolism Ribonuclease III/metabolism Schizosaccharomyces/physiology Schizosaccharomyces pombe Proteins/genetics,metabolism Transcription, Genetic/physiology
Chemicals
Chromatin RNA, Small Interfering Schizosaccharomyces pombe Proteins RNA Polymerase II Rpb7 protein, S pombe Ribonuclease III
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Djupedal Ingela
Karolinska Institutet, Department of Biosciences, School of Life Sciences, University College Sodertorn, S-141 89 Huddinge, Sweden.
Portoso Manuela
Spåhr Henrik
Bonilla Carolina
Gustafsson Claes M
Allshire Robin C
Ekwall Karl
References (34)
34 references, click to expand
  1. RNA polymerase II is required for RNAi-dependent heterochromatin assembly.
    Science. 2005 Jul 15;309(5733):467-9 PMID: 15947136
  2. RNA-interference-directed chromatin modification coupled to RNA polymerase II transcription.
    Nature. 2005 Jun 30;435(7046):1275-9 PMID: 15965464
  3. RNAi: double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals.
    Cell. 2000 Mar 31;101(1):25-33 PMID: 10778853
  4. Live analysis of lagging chromosomes during anaphase and their effect on spindle elongation rate in fission yeast.
    J Cell Sci. 2000 Dec;113 Pt 23:4177-91 PMID: 11069763
  5. Role for a bidentate ribonuclease in the initiation step of RNA interference.
    Nature. 2001 Jan 18;409(6818):363-6 PMID: 11201747
  6. Structure of an archaeal homolog of the eukaryotic RNA polymerase II RPB4/RPB7 complex.
    Mol Cell. 2001 Nov;8(5):1137-43 PMID: 11741548
  7. Requirement of heterochromatin for cohesion at centromeres.
    Science. 2001 Dec 21;294(5551):2539-42 PMID: 11598266
  8. RNAi related mechanisms affect both transcriptional and posttranscriptional transgene silencing in Drosophila.
    Mol Cell. 2002 Feb;9(2):315-27 PMID: 11864605
  9. Regulation of heterochromatic silencing and histone H3 lysine-9 methylation by RNAi.
    Science. 2002 Sep 13;297(5588):1833-7 PMID: 12193640
  10. Small RNAs correspond to centromere heterochromatic repeats.
    Science. 2002 Sep 13;297(5588):1831 PMID: 12193644
  11. Analysis of a piwi-related gene implicates small RNAs in genome rearrangement in tetrahymena.
    Cell. 2002 Sep 20;110(6):689-99 PMID: 12297043
  12. Dicer is required for chromosome segregation and gene silencing in fission yeast cells.
    Proc Natl Acad Sci U S A. 2002 Dec 24;99(26):16648-53 PMID: 12482946
  13. A role for chromatin remodeling in transcriptional termination by RNA polymerase II.
    Mol Cell. 2002 Dec;10(6):1441-52 PMID: 12504018
  14. RNA interference machinery regulates chromosome dynamics during mitosis and meiosis in fission yeast.
    Proc Natl Acad Sci U S A. 2003 Jan 7;100(1):193-8 PMID: 12509501
  15. ARGONAUTE4 control of locus-specific siRNA accumulation and DNA and histone methylation.
    Science. 2003 Jan 31;299(5607):716-9 PMID: 12522258
  16. Centromeres and kinetochores: from epigenetics to mitotic checkpoint signaling.
    Cell. 2003 Feb 21;112(4):407-21 PMID: 12600307
  17. RNA interference is required for normal centromere function in fission yeast.
    Chromosome Res. 2003;11(2):137-46 PMID: 12733640
  18. Rpb7 subunit of RNA polymerase II interacts with an RNA-binding protein involved in processing of transcripts.
    Nucleic Acids Res. 2003 Aug 15;31(16):4696-701 PMID: 12907709
  19. Mediator influences Schizosaccharomyces pombe RNA polymerase II-dependent transcription in vitro.
    J Biol Chem. 2003 Dec 19;278(51):51301-6 PMID: 14534314
  20. A DNA microarray for fission yeast: minimal changes in global gene expression after temperature shift.
    Yeast. 2004 Jan 15;21(1):25-39 PMID: 14745780
  21. RNAi-mediated targeting of heterochromatin by the RITS complex.
    Science. 2004 Jan 30;303(5658):672-6 PMID: 14704433
  22. Dicer is essential for formation of the heterochromatin structure in vertebrate cells.
    Nat Cell Biol. 2004 Aug;6(8):784-91 PMID: 15247924
  23. High-resolution protein-DNA contacts for the yeast RNA polymerase II general transcription machinery.
    Biochemistry. 2004 Oct 12;43(40):12741-9 PMID: 15461446
  24. MicroRNA genes are transcribed by RNA polymerase II.
    EMBO J. 2004 Oct 13;23(20):4051-60 PMID: 15372072
  25. Two dissociable subunits of yeast RNA polymerase II stimulate the initiation of transcription at a promoter in vitro.
    J Biol Chem. 1991 Jan 5;266(1):71-5 PMID: 1985924
  26. Comparison of Schizosaccharomyces pombe expression systems.
    Nucleic Acids Res. 1993 Jun 25;21(12):2955-6 PMID: 8332516
  27. Mutations derepressing silent centromeric domains in fission yeast disrupt chromosome segregation.
    Genes Dev. 1995 Jan 15;9(2):218-33 PMID: 7851795
  28. Gene organization and protein sequence of the small subunits of Schizosaccharomyces pombe RNA polymerase II.
    Gene. 1997 Sep 1;196(1-2):165-74 PMID: 9322754
  29. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans.
    Nature. 1998 Feb 19;391(6669):806-11 PMID: 9486653
  30. Rpb4 and Rpb7: subunits of RNA polymerase II and beyond.
    Trends Biochem Sci. 2004 Dec;29(12):674-81 PMID: 15544954
  31. Dicer-deficient mouse embryonic stem cells are defective in differentiation and centromeric silencing.
    Genes Dev. 2005 Feb 15;19(4):489-501 PMID: 15713842
  32. Plant nuclear RNA polymerase IV mediates siRNA and DNA methylation-dependent heterochromatin formation.
    Cell. 2005 Mar 11;120(5):613-22 PMID: 15766525
  33. RNA polymerase IV directs silencing of endogenous DNA.
    Science. 2005 Apr 1;308(5718):118-20 PMID: 15692015
  34. Fission yeast mutants that alleviate transcriptional silencing in centromeric flanking repeats and disrupt chromosome segregation.
    Genetics. 1999 Nov;153(3):1153-69 PMID: 10545449
Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.344205
Published
2005-10-01
Pages
2301-6
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC1240039
Subset
IM
Grants
Wellcome Trust · United Kingdom
External Links
PubMed source DOI: 10.1101/gad.344205 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2