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PMID: 16260474 Published · epublish English Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, P.H.S.

Identification of RecQL1 as a Holliday junction processing enzyme in human cell lines.

Nucleic acids research ·Vol. 33 ·No. 19 ·2005-00-00 ·Pages 6251-7

LeRoy G, Carroll R, Kyin S, Seki M, Cole MD

Abstract

Homologous recombination provides an effective way to repair DNA double-strand breaks (DSBs) and is required for genetic recombination. During the process of homologous recombination, a heteroduplex DNA structure, or a 'Holliday junction' (HJ), is formed. The movement, or branch migration, of this junction is necessary for recombination to proceed correctly. In prokaryotes, the RecQ protein or the RuvA/RuvB protein complex can promote ATP-dependent branch migration of Holliday junctions. Much less is known about the processing of Holliday junctions in eukaryotes. Here, we identify RecQL1 as a predominant ATP-dependent, HJ branch migrator present in human nuclear extracts. A reduction in the level of RecQL1 induced by RNA interference in HeLa cells leads to an increase in sister chromatid exchange. We propose that RecQL1 is involved in the processing of Holliday junctions in human cells.

MeSH Terms
Adenosine Triphosphatases/antagonists & inhibitors,genetics,metabolism Adenosine Triphosphate/metabolism Cell Line Cell Nucleus/enzymology DNA Helicases/antagonists & inhibitors,genetics,metabolism DNA, Cruciform/metabolism HeLa Cells Humans RNA Interference RecQ Helicases Sister Chromatid Exchange
Chemicals
DNA, Cruciform Adenosine Triphosphate Adenosine Triphosphatases RECQL4 protein, human DNA Helicases RecQ Helicases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
LeRoy Gary
Department of Molecular Biology, Princeton University, Washington Road, Princeton, NJ 08544-1014, USA. [email protected]
Carroll Robert
Kyin Saw
Seki Masayuki
Cole Michael D
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
1362-4962
DOI
10.1093/nar/gki929
Email
[email protected]
Published
2005-00-00
Epub
2005-00-31
Pages
6251-7
Language
English
Region
England
NLM ID
0411011
PMCID
PMC1275589
Subset
IM
Grants
NCI NIH HHS · R01 CA055248 · United States
NCI NIH HHS · R01 CA080320 · United States
External Links
PubMed source DOI: 10.1093/nar/gki929 PMC Full Text View journal details
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