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PMID: 16280388 Published · ppublish English Comparative Study Journal Article Research Support, N.I.H., Extramural

Activation-induced deaminase cloning, localization, and protein extraction from young VH-mutant rabbit appendix.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 102 ·No. 47 ·2005-11-22 ·Pages 17083-8

Yang G, Obiakor H, Sinha RK, Newman BA, Hood BL, Conrads TP, Veenstra TD, Mage RG

Abstract

Studies in mouse, human, and chicken suggest that activation-induced deaminase (AID) is involved in three known processes leading to antibody diversification: somatic hypermutation, gene conversion, and class-switch recombination. Developing rabbit appendix provides a particularly good site for studying all three of these B cell maturation events. We report here successful cloning of rabbit AID and isolation of AID protein from rabbit appendix-cell nuclear and cytoplasmic extracts. We succeeded in identifying and locating AID protein in cells by immunohistochemical and immunofluorescent staining techniques and examined colocalization of AID and other molecules important for Ab diversification. This report extends our knowledge about AID to a mammalian species that uses gene conversion to diversify rearranged Ig genes. Although much work remains to understand fully the mechanism of action of AID and its association with other cellular components, the rabbit system now offers a particularly useful model for future studies of these dynamics.

MeSH Terms
Amino Acid Sequence Animals Appendix/enzymology Base Sequence Blotting, Western Cell Division/physiology Chickens Cytidine Deaminase/genetics,isolation & purification,metabolism Fluorescent Antibody Technique Humans Immunoglobulin Heavy Chains/genetics Immunoglobulin Variable Region/genetics Immunohistochemistry Mice Molecular Sequence Data Mutation Rabbits Sequence Alignment
Chemicals
Immunoglobulin Heavy Chains Immunoglobulin Variable Region AICDA (activation-induced cytidine deaminase) Cytidine Deaminase
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Yang Guibin
Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, and SAIC Frederick, National Cancer Institute, Frederick, MD 21701, USA.
Obiakor Harold
Sinha Rajesh K
Newman Barbara A
Hood Brian L
Conrads Thomas P
Veenstra Timothy D
Mage Rose G
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.0501338102
Published
2005-11-22
Epub
2005-00-09
Pages
17083-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC1282565
Subset
IM
Grants
NCI NIH HHS · N01CO12400 · United States
NCI NIH HHS · N01 CO 12400 · United States
Databases
GENBANK
AY928183
External Links
PubMed source DOI: 10.1073/pnas.0501338102 PMC Full Text View journal details
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