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PMID: 16301175 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Ephrin-as guide the formation of functional maps in the visual cortex.

Neuron ·Vol. 48 ·No. 4 ·2005-11-23 ·Pages 577-89

Cang J, Kaneko M, Yamada J, Woods G, Stryker MP, Feldheim DA

Abstract

Ephrin-As and their receptors, EphAs, are expressed in the developing cortex where they may act to organize thalamic inputs. Here, we map the visual cortex (V1) in mice deficient for ephrin-A2, -A3, and -A5 functionally, using intrinsic signal optical imaging and microelectrode recording, and structurally, by anatomical tracing of thalamocortical projections. V1 is shifted medially, rotated, and compressed and its internal organization is degraded. Expressing ephrin-A5 ectopically by in utero electroporation in the lateral cortex shifts the map of V1 medially, and expression within V1 disrupts its internal organization. These findings indicate that interactions between gradients of EphA/ephrin-A in the cortex guide map formation, but that factors other than redundant ephrin-As are responsible for the remnant map. Together with earlier work on the retinogeniculate map, the current findings show that the same molecular interactions may operate at successive stages of the visual pathway to organize maps.

MeSH Terms
Aging/metabolism,physiology Animals Animals, Newborn Brain Mapping Embryonic Development Ephrin-A2/deficiency,metabolism,physiology Ephrin-A3/deficiency,metabolism,physiology Ephrin-A5/deficiency,metabolism,physiology Ligands Mice Mice, Knockout Retina/physiology Synaptic Transmission/physiology Thalamus/embryology,growth & development,physiology Visual Cortex/embryology,growth & development,physiology
Chemicals
Ephrin-A2 Ephrin-A3 Ephrin-A5 Ligands
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Cang Jianhua
W. M. Keck Foundation Center for Integrative Neuroscience, Department of Physiology, University of California, San Francisco, San Francisco, California 94143, USA.
Kaneko Megumi
Yamada Jena
Woods Georgia
Stryker Michael P
Feldheim David A
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Article Info
Journal
Neuron
Abbr.
Neuron
ISSN
0896-6273
DOI
10.1016/j.neuron.2005.10.026
Published
2005-11-23
Pages
577-89
Language
English
Region
United States
NLM ID
8809320
PMCID
PMC2424263
Subset
IM
Grants
NEI NIH HHS · EY014689 · United States
NEI NIH HHS · R01 EY002874 · United States
NEI NIH HHS · R37 EY002874-24S1 · United States
NEI NIH HHS · EY02874 · United States
NEI NIH HHS · R37 EY002874 · United States
NEI NIH HHS · R01 EY014689 · United States
Corrections
CommentIn
PMID: 16301165
External Links
PubMed source DOI: 10.1016/j.neuron.2005.10.026 PMC Full Text View journal details
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