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PMID: 16314430 Published · ppublish English Journal Article

Mutants in trs120 disrupt traffic from the early endosome to the late Golgi.

The Journal of cell biology ·Vol. 171 ·No. 5 ·2005-12-05 ·Pages 823-33

Cai H, Zhang Y, Pypaert M, Walker L, Ferro-Novick S

Abstract

Transport protein particle (TRAPP), a large complex that mediates membrane traffic, is found in two forms (TRAPPI and -II). Both complexes share seven subunits, whereas three subunits (Trs130p, -120p, and -65p) are specific to TRAPPII. Previous studies have shown that mutations in the TRAPPII-specific gene trs130 block traffic through or from the Golgi. Surprisingly, we report that mutations in trs120 do not block general secretion. Instead, trs120 mutants accumulate aberrant membrane structures that resemble Berkeley bodies and disrupt the traffic of proteins that recycle through the early endosome. Mutants defective in recycling also display a defect in the localization of coat protein I (COPI) subunits, implying that Trs120p may participate in a COPI-dependent trafficking step on the early endosomal pathway. Furthermore, we demonstrate that Trs120p largely colocalizes with the late Golgi marker Sec7p. Our findings imply that Trs120p is required for vesicle traffic from the early endosome to the late Golgi.

MeSH Terms
Coat Protein Complex I/metabolism Endosomes/genetics,metabolism,ultrastructure Golgi Apparatus/genetics,metabolism,ultrastructure Guanine Nucleotide Exchange Factors/metabolism Membrane Proteins/genetics,physiology Microscopy, Electron, Transmission Mutation Protein Subunits/genetics,physiology Protein Transport Saccharomyces cerevisiae/genetics,metabolism,ultrastructure Saccharomyces cerevisiae Proteins/genetics,physiology Vesicular Transport Proteins/genetics,physiology
Chemicals
Coat Protein Complex I Guanine Nucleotide Exchange Factors Membrane Proteins Protein Subunits Saccharomyces cerevisiae Proteins Sec7 guanine nucleotide exchange factors TRS130 protein, S cerevisiae Vesicular Transport Proteins transport protein particle, TRAPP
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Cai Huaqing
Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT 06519, USA.
Zhang Yueyi
Pypaert Marc
Walker Lee
Ferro-Novick Susan
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200505145
Published
2005-12-05
Epub
2005-00-28
Pages
823-33
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2171297
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.200505145 PMC Full Text View journal details
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