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PMID: 16321968 Published · epublish English Journal Article Research Support, Non-U.S. Gov't

A haploid-specific transcriptional response to irradiation in Saccharomyces cerevisiae.

Nucleic acids research ·Vol. 33 ·No. 20 ·2005-00-00 ·Pages 6635-43

Mercier G, Berthault N, Touleimat N, Képès F, Fourel G, Gilson E, Dutreix M

Abstract

Eukaryotic cells respond to DNA damage by arresting the cell cycle and modulating gene expression to ensure efficient DNA repair. We used global transcriptome analysis to investigate the role of ploidy and mating-type in inducing the response to damage in various Saccharomyces cerevisiae strains. We observed a response to DNA damage specific to haploid strains that seemed to be controlled by chromatin regulatory proteins. Consistent with these microarray data, we found that mating-type factors controlled the chromatin-dependent silencing of a reporter gene. Both these analyses demonstrate the existence of an irradiation-specific response in strains (haploid or diploid) with only one mating-type factor. This response depends on the activities of Hdf1 and Sir2. Overall, our results suggest the existence of a new regulation pathway dependent on mating-type factors, chromatin structure remodeling, Sir2 and Hdf1 and independent of Mec1 kinase.

MeSH Terms
Antigens, Nuclear/physiology Chromatin/metabolism Chromosomes, Bacterial DNA Damage DNA Repair DNA-Binding Proteins/physiology Diploidy Gene Expression Regulation, Bacterial Gene Silencing Genes, Bacterial Haploidy Histone Deacetylases/physiology Ku Autoantigen Promoter Regions, Genetic Saccharomyces cerevisiae/genetics,metabolism,radiation effects Silent Information Regulator Proteins, Saccharomyces cerevisiae/physiology Sirtuin 2 Sirtuins/physiology Transcription, Genetic/radiation effects
Chemicals
Antigens, Nuclear Chromatin DNA-Binding Proteins Silent Information Regulator Proteins, Saccharomyces cerevisiae SIR2 protein, S cerevisiae Sirtuin 2 Sirtuins Histone Deacetylases Ku Autoantigen
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Mercier G
CNRS-UMR 2027, Institut Curie, Bât. 110, Centre Universitaire, F-91405 Orsay, France.
Berthault N
Touleimat N
Képès F
Fourel G
Gilson E
Dutreix M
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
1362-4962
DOI
10.1093/nar/gki959
Published
2005-00-00
Epub
2005-00-30
Pages
6635-43
Language
English
Region
England
NLM ID
0411011
PMCID
PMC1298924
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/gki959 PMC Full Text View journal details
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