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PMID: 16326926 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

pTAC2, -6, and -12 are components of the transcriptionally active plastid chromosome that are required for plastid gene expression.

The Plant cell ·Vol. 18 ·No. 1 ·2006-01-00 ·Pages 176-97

Pfalz J, Liere K, Kandlbinder A, Dietz KJ, Oelmüller R

Abstract

Transcription in plastids is mediated by a plastid-encoded multimeric (PEP) and a nuclear-encoded single-subunit RNA polymerase (NEP) and a still unknown number of nuclear-encoded factors. By combining gel filtration and affinity chromatography purification steps, we isolated transcriptionally active chromosomes from Arabidopsis thaliana and mustard (Sinapis alba) chloroplasts and identified 35 components by electrospray ionization ion trap tandem mass spectrometry. Eighteen components, called plastid transcriptionally active chromosome proteins (pTACs), have not yet been described. T-DNA insertions in three corresponding genes, ptac2, -6, and -12, are lethal without exogenous carbon sources. Expression patterns of the plastid-encoded genes in the corresponding knockout lines resemble those of Deltarpo mutants. For instance, expression of plastid genes with PEP promoters is downregulated, while expression of genes with NEP promoters is either not affected or upregulated in the mutants. All three components might also be involved in posttranscriptional processes, such as RNA processing and/or mRNA stability. Thus, pTAC2, -6, and -12 are clearly involved in plastid gene expression.

MeSH Terms
Arabidopsis/genetics,metabolism Chromosomes, Plant/metabolism DNA-Directed RNA Polymerases/genetics,metabolism Gene Expression Gene Expression Regulation, Plant Molecular Sequence Data Mustard Plant/genetics,metabolism Mutation Nuclear Proteins/genetics,metabolism Oligonucleotide Array Sequence Analysis Phenotype Plant Proteins/genetics,metabolism Plants, Genetically Modified Plastids/genetics,metabolism,ultrastructure Promoter Regions, Genetic Transcription, Genetic
Chemicals
Nuclear Proteins Plant Proteins DNA-Directed RNA Polymerases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Pfalz Jeannette
Institute of General Botany and Plant Physiology, Friedrich-Schiller-University, Jena, Germany.
Liere Karsten
Kandlbinder Andrea
Dietz Karl-Josef
Oelmüller Ralf
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.105.036392
Published
2006-01-00
Epub
2005-00-02
Pages
176-97
Language
English
Region
England
NLM ID
9208688
PMCID
PMC1323492
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.105.036392 PMC Full Text View journal details
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