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PMID: 16344568 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Variation in crossing-over rates across chromosome 4 of Arabidopsis thaliana reveals the presence of meiotic recombination "hot spots".

Genome research ·Vol. 16 ·No. 1 ·2006-01-00 ·Pages 106-14

Drouaud J, Camilleri C, Bourguignon PY, Canaguier A, Bérard A, Vezon D, Giancola S, Brunel D, Colot V, Prum B, Quesneville H, Mézard C

Abstract

Crossover (CO) is a key process for the accurate segregation of homologous chromosomes during the first meiotic division. In most eukaryotes, meiotic recombination is not homogeneous along the chromosomes, suggesting a tight control of the location of recombination events. We genotyped 71 single nucleotide polymorphisms (SNPs) covering the entire chromosome 4 of Arabidopsis thaliana on 702 F2 plants, representing 1404 meioses and allowing the detection of 1171 COs, to study CO localization in a higher plant. The genetic recombination rates varied along the chromosome from 0 cM/Mb near the centromere to 20 cM/Mb on the short arm next to the NOR region, with a chromosome average of 4.6 cM/Mb. Principal component analysis showed that CO rates negatively correlate with the G+C content (P = 3x10(-4)), in contrast to that reported in other eukaryotes. COs also significantly correlate with the density of single repeats and the CpG ratio, but not with genes, pseudogenes, transposable elements, or dispersed repeats. Chromosome 4 has, on average, 1.6 COs per meiosis, and these COs are subjected to interference. A detailed analysis of several regions having high CO rates revealed "hot spots" of meiotic recombination contained in small fragments of a few kilobases. Both the intensity and the density of these hot spots explain the variation of CO rates along the chromosome.

MeSH Terms
Arabidopsis/genetics Base Composition/genetics Centromere/genetics Chromosomes, Plant/genetics Crossing Over, Genetic/genetics Genetic Variation Meiosis/genetics Polymorphism, Single Nucleotide Repetitive Sequences, Nucleic Acid/genetics
Authors & Affiliations
12 authors, click to expand affiliations / ORCID
Drouaud Jan
Station de Génétique et d'Amélioration des Plantes, Institut Jean-Pierre Bourgin, Institut National de la Recherche Agronomique, 78026, Versailles cedex, France.
Camilleri Christine
Bourguignon Pierre-Yves
Canaguier Aurélie
Bérard Aurélie
Vezon Daniel
Giancola Sandra
Brunel Dominique
Colot Vincent
Prum Bernard
Quesneville Hadi
Mézard Christine
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Article Info
Journal
Genome research
Abbr.
Genome Res
ISSN
1088-9051
DOI
10.1101/gr.4319006
Published
2006-01-00
Epub
2005-00-12
Pages
106-14
Language
English
Region
United States
NLM ID
9518021
PMCID
PMC1356134
Subset
IM
External Links
PubMed source DOI: 10.1101/gr.4319006 PMC Full Text View journal details
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