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PMID: 16377758 Published · ppublish English Journal Article

The Arabidopsis receptor kinase FLS2 binds flg22 and determines the specificity of flagellin perception.

The Plant cell ·Vol. 18 ·No. 2 ·2006-02-00 ·Pages 465-76

Chinchilla D, Bauer Z, Regenass M, Boller T, Felix G

Abstract

Flagellin, the main building block of the bacterial flagellum, acts as a pathogen-associated molecular pattern triggering the innate immune response in animals and plants. In Arabidopsis thaliana, the Leu-rich repeat transmembrane receptor kinase FLAGELLIN SENSITIVE2 (FLS2) is essential for flagellin perception. Here, we demonstrate the specific interaction of the elicitor-active epitope flg22 with the FLS2 protein by chemical cross-linking and immunoprecipitation. The functionality of this receptor was further tested by heterologous expression of the Arabidopsis FLS2 gene in tomato (Lycopersicon esculentum) cells. The perception of flg22 in tomato differs characteristically from that in Arabidopsis. Expression of Arabidopsis FLS2 conferred an additional flg22-perception system on the cells of tomato, which showed all of the properties characteristic of the perception of this elicitor in Arabidopsis. In summary, these results show that FLS2 constitutes the pattern-recognition receptor that determines the specificity of flagellin perception.

MeSH Terms
Alkalies/analysis Antibodies/immunology Arabidopsis/cytology,immunology,metabolism,microbiology Arabidopsis Proteins/analysis,chemistry,immunology,metabolism Binding Sites Binding, Competitive Cross-Linking Reagents Flagellin/chemistry,immunology,metabolism Gene Expression Immunoprecipitation Iodine Radioisotopes Lycopersicon esculentum/cytology,immunology,metabolism Molecular Weight Mutation/genetics Plant Diseases/microbiology Protein Binding Protein Kinases/analysis,chemistry,immunology,metabolism Substrate Specificity
Chemicals
Alkalies Antibodies Arabidopsis Proteins Cross-Linking Reagents Iodine Radioisotopes Flagellin Protein Kinases FLS2 protein, Arabidopsis
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Chinchilla Delphine
Zürich-Basel Plant Science Center, Botanisches Institut der Universität Basel, CH-4056 Basel, Switzerland.
Bauer Zsuzsa
Regenass Martin
Boller Thomas
Felix Georg
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.105.036574
Published
2006-02-00
Epub
2005-00-23
Pages
465-76
Language
English
Region
England
NLM ID
9208688
PMCID
PMC1356552
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.105.036574 PMC Full Text View journal details
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