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PMID: 163792 Published · ppublish English Journal Article

Escherichia coli enterotoxin: stimulation of adenylate cyclase in broken-cell preparations.

Infection and immunity ·Vol. 11 ·No. 3 ·1975-03-00 ·Pages 429-35

Dorner F, Mayer P

Abstract

The enterotoxin from cell-free filtrates of the enteropathogenic Escherichia coli strain P-263 was found to stimulate adenylate cyclase activity in broken-cell preparations from myocardial tissue. Particulate and detergent-solubilized fractions from cat heart were incubated with enterotoxin and assayed for adenylate cyclase activity. Adenylate cyclase activity was stimulated by enterotoxin; the extent of stimulation was proportional to the concentration of enterotoxin. The data demonstrate that stimulation of enterotoxin-sensitive adenylate cyclase in this system provides a sensitive in vitro assay, either as an accurate measure of enterotoxin concentration or as an assay for antitoxic titers in sera. A parallel comparison showed that stimulation of fluid production in rabbit intestinal loops by enterotoxin was less sensitive.

MeSH Terms
Adenylyl Cyclases/metabolism Animals Antitoxins Cats Cyclic AMP/metabolism Detergents Enterotoxins/isolation & purification,pharmacology Escherichia coli/immunology Goats/immunology Hot Temperature Intestines/immunology Myocardium/enzymology Neutralization Tests Phosphatidylserines Phosphoric Diester Hydrolases/metabolism Rabbits/immunology Radioisotope Dilution Technique Theophylline Thyroxine Time Factors Tritium Venoms
Chemicals
Antitoxins Detergents Enterotoxins Phosphatidylserines Venoms Tritium Theophylline Cyclic AMP Phosphoric Diester Hydrolases Adenylyl Cyclases Thyroxine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Dorner F
Mayer P
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22 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.11.3.429-435.1975
Published
1975-03-00
Pages
429-35
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC415081
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.11.3.429-435.1975 PMC Full Text View journal details
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