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PMID: 4318781 Published · ppublish English Journal Article

A protein binding assay for adenosine 3':5'-cyclic monophosphate.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 67 ·No. 1 ·1970-09-00 ·Pages 305-12

Gilman AG

Abstract

A simple and sensitive assay for adenosine 3':5'-cyclic monophosphate (cAMP) has been developed that is based on competition for protein binding of the nucleotide, presumably to a cAMP-dependent protein kinase. The nucleotide-protein complex is adsorbed on a cellulose ester filter. Assay conditions are such that a binding constant approaching 10(-9) M is obtained, and the assay is thus sensitive to 0.05-0.10 pmol of cAMP.

MeSH Terms
Adenine Nucleotides/analysis Animals Brain Chemistry Cattle Cellulose Chromatography, DEAE-Cellulose Cyclic AMP/analysis Filtration Hydrogen-Ion Concentration Kinetics Liver/analysis Methods Mice Muscles/enzymology Phosphotransferases Protein Binding Proteins Tritium
Chemicals
Adenine Nucleotides Proteins Tritium Cellulose Cyclic AMP Phosphotransferases
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Gilman A G
References (19)
19 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.67.1.305
Published
1970-09-00
Pages
305-12
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC283204
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.67.1.305 PMC Full Text View journal details
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