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PMID: 16428443 Published · ppublish English Journal Article

Host cell factor and an uncharacterized SANT domain protein are stable components of ATAC, a novel dAda2A/dGcn5-containing histone acetyltransferase complex in Drosophila.

Molecular and cellular biology ·Vol. 26 ·No. 3 ·2006-02-00 ·Pages 871-82

Guelman S, Suganuma T, Florens L, Swanson SK, Kiesecker CL, Kusch T, Anderson S, Yates JR, Washburn MP, Abmayr SM, Workman JL

Abstract

Gcn5 is a conserved histone acetyltransferase (HAT) found in a number of multisubunit complexes from Saccharomyces cerevisiae, mammals, and flies. We previously identified Drosophila melanogaster homologues of the yeast proteins Ada2, Ada3, Spt3, and Tra1 and showed that they associate with dGcn5 to form at least two distinct HAT complexes. There are two different Ada2 homologues in Drosophila named dAda2A and dAda2B. dAda2B functions within the Drosophila version of the SAGA complex (dSAGA). To gain insight into dAda2A function, we sought to identify novel components of the complex containing this protein, ATAC (Ada two A containing) complex. Affinity purification and mass spectrometry revealed that, in addition to dAda3 and dGcn5, host cell factor (dHCF) and a novel SANT domain protein, named Atac1 (ATAC component 1), copurify with this complex. Coimmunoprecipitation experiments confirmed that these proteins associate with dGcn5 and dAda2A, but not with dSAGA-specific components such as dAda2B and dSpt3. Biochemical fractionation revealed that ATAC has an apparent molecular mass of 700 kDa and contains dAda2A, dGcn5, dAda3, dHCF, and Atac1 as stable subunits. Thus, ATAC represents a novel histone acetyltransferase complex that is distinct from previously purified Gcn5/Pcaf-containing complexes from yeast and mammalian cells.

MeSH Terms
Amino Acid Sequence Animals Antibodies/immunology Chromosomes/chemistry Drosophila Proteins/analysis,immunology,metabolism Drosophila melanogaster/metabolism Histone Acetyltransferases/analysis,metabolism Immunoprecipitation Molecular Sequence Data
Chemicals
Ada2a protein, Drosophila Antibodies Atac1 protein, Drosophila Drosophila Proteins host cell factor, Drosophila ADA3 protein, Drosophila GCN5 protein, Drosophila Histone Acetyltransferases
Authors & Affiliations
11 authors, click to expand affiliations / ORCID
Guelman Sebastián
Stowers Institute for Medical Research, 1000 E. 50th St., Kansas City, MO 64110, USA.
Suganuma Tamaki
Florens Laurence
Swanson Selene K
Kiesecker Cheri L
Kusch Thomas
Anderson Scott
Yates John R
Washburn Michael P
Abmayr Susan M
Workman Jerry L
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.26.3.871-882.2006
Published
2006-02-00
Pages
871-82
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC1347012
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.26.3.871-882.2006 PMC Full Text View journal details
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