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PMID: 16436510 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Review

Kiss-and-coat and compartment mixing: coupling exocytosis to signal generation and local actin assembly.

Molecular biology of the cell ·Vol. 17 ·No. 4 ·2006-04-00 ·Pages 1495-502

Sokac AM, Bement WM

Abstract

Regulated exocytosis is thought to occur either by "full fusion," where the secretory vesicle fuses with the plasma membrane (PM) via a fusion pore that then dilates until the secretory vesicle collapses into the PM; or by "kiss-and-run," where the fusion pore does not dilate and instead rapidly reseals such that the secretory vesicle is retrieved almost fully intact. Here, we describe growing evidence for a third form of exocytosis, dubbed "kiss-and-coat," which is characteristic of a broad variety of cell types that undergo regulated exocytosis. Kiss-and-coat exocytosis entails prolonged maintenance of a dilated fusion pore and assembly of actin filament (F-actin) coats around the exocytosing secretory vesicles followed by direct retrieval of some fraction of the emptied vesicle membrane. We propose that assembly of the actin coats results from the union of the secretory vesicle membrane and PM and that this compartment mixing represents a general mechanism for generating local signals via directed membrane fusion.

MeSH Terms
Actins/analysis,metabolism Animals Cell Membrane/chemistry,metabolism Exocytosis Membrane Fusion Secretory Vesicles/chemistry,metabolism Signal Transduction
Chemicals
Actins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sokac Anna M
Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.
Bement William M
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.e05-10-0908
Published
2006-04-00
Epub
2006-00-25
Pages
1495-502
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC1415325
Subset
IM
Grants
NIGMS NIH HHS · R01 GM052932 · United States
NIGMS NIH HHS · GM 52932 · United States
External Links
PubMed source DOI: 10.1091/mbc.e05-10-0908 PMC Full Text View journal details
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