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PMID: 16453562 Published · ppublish English Journal Article

Delivery of T-DNA from the Agrobacterium tumefaciens chromosome into plant cells.

The EMBO journal ·Vol. 3 ·No. 11 ·1984-11-00 ·Pages 2485-90

Hoekema A, Roelvink PW, Hooykaas PJ, Schilperoort RA

Abstract

The intact T-region of the B6Ti plasmid of Agrobacterium tumefaciens was stepwise cloned into a site in transposon Tn3. In this way a suitable vehicle (Tn1882) was obtained for translocating the T-region to different replicons, i.e., to other plasmids or the chromosome. The IncP plasmid R772::Tn1882 conferred tumorigenicity on Agrobacterium if the virulence genes were provided in trans in the same cell. This result showed that the T-region present on Tn1882 was transferred efficiently to plant cells. Normal tumor development also occurred if the T-region was placed in the chromosome of A. tumefaciens and an R' plasmid was present carrying virA-E or virA-F. We conclude that the plasmid location of the T-region is not a prerequisite for transfer to the plant cell. The apparently normal delivery of the T-DNA from a bacterial chromosomal location supports a model involving a processing step within Agrobacterium effecting transfer of the T-region as a separate entity.

Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hoekema A
Molbas Research Group, Department of Plant Molecular Biology, University of Leiden, Wassenaarseweg 64, 2333 AL Leiden, The Netherlands.
Roelvink P W
Hooykaas P J
Schilperoort R A
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1984-11-00
Pages
2485-90
Language
English
Region
England
NLM ID
8208664
PMCID
PMC557716
External Links
PubMed source PMC Full Text View journal details
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