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PMID: 16453690 Published · ppublish English Journal Article

Primary structure of a developmentally regulated nicotinic acetylcholine receptor protein from Drosophila.

The EMBO journal ·Vol. 5 ·No. 7 ·1986-07-00 ·Pages 1503-8

Hermans-Borgmeyer I, Zopf D, Ryseck RP, Hovemann B, Betz H, Gundelfinger ED

Abstract

Acetylcholine is a major excitatory neurotransmitter in the central nervous system of insects. Using DNA probes of the Torpedo nicotinic acetylcholine receptor (AChR) we have isolated two overlapping cDNA clones encoding a putative neuronal AChR protein from the fruitfly, Drosophila melanogaster. The predicted mature protein consists of 497 amino acids, has a calculated mol. wt of 57 340 and shows extensive homology to known AChR subunits from different species along its entire amino acid sequence. Northern analysis revealed a hybridizing mRNA of 3.2 kb in late embryo and in pupae. Expression of the corresponding AChR gene thus characterizes periods of neuronal differentiation in Drosophila.

Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Hermans-Borgmeyer I
Zentrum für Molekulare Biologie, Universität Heidelberg, Im Neuenheimer Feld 282, D-6900 Heidelberg, FRG.
Zopf D
Ryseck R P
Hovemann B
Betz H
Gundelfinger E D
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1986-07-00
Pages
1503-8
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1166972
External Links
PubMed source PMC Full Text View journal details
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