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PMID: 16461460 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

A microRNA expression signature of human solid tumors defines cancer gene targets.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 103 ·No. 7 ·2006-02-14 ·Pages 2257-61

Volinia S, Calin GA, Liu CG, Ambs S, Cimmino A, Petrocca F, Visone R, Iorio M, Roldo C, Ferracin M, Prueitt RL, Yanaihara N, Lanza G, Scarpa A, Vecchione A, Negrini M, Harris CC, Croce CM

Abstract

Small noncoding microRNAs (miRNAs) can contribute to cancer development and progression and are differentially expressed in normal tissues and cancers. From a large-scale miRnome analysis on 540 samples including lung, breast, stomach, prostate, colon, and pancreatic tumors, we identified a solid cancer miRNA signature composed by a large portion of overexpressed miRNAs. Among these miRNAs are some with well characterized cancer association, such as miR-17-5p, miR-20a, miR-21, miR-92, miR-106a, and miR-155. The predicted targets for the differentially expressed miRNAs are significantly enriched for protein-coding tumor suppressors and oncogenes (P < 0.0001). A number of the predicted targets, including the tumor suppressors RB1 (Retinoblastoma 1) and TGFBR2 (transforming growth factor, beta receptor II) genes were confirmed experimentally. Our results indicate that miRNAs are extensively involved in cancer pathogenesis of solid tumors and support their function as either dominant or recessive cancer genes.

MeSH Terms
Gene Expression Profiling Genes, Neoplasm/genetics Genes, Tumor Suppressor Humans MicroRNAs/genetics,metabolism Neoplasms/genetics Oligonucleotide Array Sequence Analysis Tumor Cells, Cultured
Chemicals
MicroRNAs
Authors & Affiliations
18 authors, click to expand affiliations / ORCID
Volinia Stefano
Department of Molecular Virology, Immunology, and Medical Genetics and Cancer Comprehensive Center, Ohio State University, Columbus, OH 43210, USA.
Calin George A
Liu Chang-Gong
Ambs Stefan
Cimmino Amelia
Petrocca Fabio
Visone Rosa
Iorio Marilena
Roldo Claudia
Ferracin Manuela
Prueitt Robyn L
Yanaihara Nozumu
Lanza Giovanni
Scarpa Aldo
Vecchione Andrea
Negrini Massimo
Harris Curtis C
Croce Carlo M
References (29)
29 references, click to expand
  1. miRNAs, cancer, and stem cell division.
    Cell. 2005 Jul 15;122(1):6-7 PMID: 16009126
  2. c-Myc-regulated microRNAs modulate E2F1 expression.
    Nature. 2005 Jun 9;435(7043):839-43 PMID: 15944709
  3. MicroRNA gene expression deregulation in human breast cancer.
    Cancer Res. 2005 Aug 15;65(16):7065-70 PMID: 16103053
  4. BIC and miR-155 are highly expressed in Hodgkin, primary mediastinal and diffuse large B cell lymphomas.
    J Pathol. 2005 Oct;207(2):243-9 PMID: 16041695
  5. miR-15 and miR-16 induce apoptosis by targeting BCL2.
    Proc Natl Acad Sci U S A. 2005 Sep 27;102(39):13944-9 PMID: 16166262
  6. A MicroRNA signature associated with prognosis and progression in chronic lymphocytic leukemia.
    N Engl J Med. 2005 Oct 27;353(17):1793-801 PMID: 16251535
  7. Restoration of TGF-beta signalling reduces tumorigenicity in human lung cancer cells.
    Br J Cancer. 2005 Nov 14;93(10):1157-67 PMID: 16251876
  8. Unique microRNA molecular profiles in lung cancer diagnosis and prognosis.
    Cancer Cell. 2006 Mar;9(3):189-98 PMID: 16530703
  9. Significance analysis of microarrays applied to the ionizing radiation response.
    Proc Natl Acad Sci U S A. 2001 Apr 24;98(9):5116-21 PMID: 11309499
  10. Diagnosis of multiple cancer types by shrunken centroids of gene expression.
    Proc Natl Acad Sci U S A. 2002 May 14;99(10):6567-72 PMID: 12011421
  11. Frequent deletions and down-regulation of micro- RNA genes miR15 and miR16 at 13q14 in chronic lymphocytic leukemia.
    Proc Natl Acad Sci U S A. 2002 Nov 26;99(24):15524-9 PMID: 12434020
  12. MicroRNA pathways in flies and worms: growth, death, fat, stress, and timing.
    Cell. 2003 Jun 13;113(6):673-6 PMID: 12809598
  13. Reduced accumulation of specific microRNAs in colorectal neoplasia.
    Mol Cancer Res. 2003 Oct;1(12):882-91 PMID: 14573789
  14. The microRNA Registry.
    Nucleic Acids Res. 2004 Jan 1;32(Database issue):D109-11 PMID: 14681370
  15. High expression of precursor microRNA-155/BIC RNA in children with Burkitt lymphoma.
    Genes Chromosomes Cancer. 2004 Feb;39(2):167-9 PMID: 14695998
  16. MicroRNAs: genomics, biogenesis, mechanism, and function.
    Cell. 2004 Jan 23;116(2):281-97 PMID: 14744438
  17. Prognostic significance of transforming growth factor beta receptor II in estrogen receptor-negative breast cancer patients.
    Clin Cancer Res. 2004 Jan 15;10(2):491-8 PMID: 14760070
  18. Reduced expression of the let-7 microRNAs in human lung cancers in association with shortened postoperative survival.
    Cancer Res. 2004 Jun 1;64(11):3753-6 PMID: 15172979
  19. An oligonucleotide microchip for genome-wide microRNA profiling in human and mouse tissues.
    Proc Natl Acad Sci U S A. 2004 Jun 29;101(26):9740-4 PMID: 15210942
  20. MicroRNA profiling reveals distinct signatures in B cell chronic lymphocytic leukemias.
    Proc Natl Acad Sci U S A. 2004 Aug 10;101(32):11755-60 PMID: 15284443
  21. RB1 protein in normal and malignant human colorectal tissue and colon cancer cell lines.
    FASEB J. 1993 Jul;7(10):931-7 PMID: 8344490
  22. Sample size calculation for multiple testing in microarray data analysis.
    Biostatistics. 2005 Jan;6(1):157-69 PMID: 15618534
  23. Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets.
    Cell. 2005 Jan 14;120(1):15-20 PMID: 15652477
  24. Accumulation of miR-155 and BIC RNA in human B cell lymphomas.
    Proc Natl Acad Sci U S A. 2005 Mar 8;102(10):3627-32 PMID: 15738415
  25. Transcription and processing of human microRNA precursors.
    Mol Cell. 2004 Dec 22;16(6):861-5 PMID: 15610730
  26. RAS is regulated by the let-7 microRNA family.
    Cell. 2005 Mar 11;120(5):635-47 PMID: 15766527
  27. A microRNA polycistron as a potential human oncogene.
    Nature. 2005 Jun 9;435(7043):828-33 PMID: 15944707
  28. MicroRNA expression profiles classify human cancers.
    Nature. 2005 Jun 9;435(7043):834-8 PMID: 15944708
  29. MicroRNA-21 is an antiapoptotic factor in human glioblastoma cells.
    Cancer Res. 2005 Jul 15;65(14):6029-33 PMID: 16024602
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.0510565103
Published
2006-02-14
Epub
2006-00-03
Pages
2257-61
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC1413718
Subset
IM
Grants
NCI NIH HHS · P30CA56036 · United States
Telethon · GTF03012 · Italy
NCI NIH HHS · P01CA76259 · United States
NCI NIH HHS · P01 CA081534 · United States
NCI NIH HHS · P01 CA076259 · United States
NCI NIH HHS · P01CA81534 · United States
NCI NIH HHS · P30 CA056036 · United States
External Links
PubMed source DOI: 10.1073/pnas.0510565103 PMC Full Text View journal details
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