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PMID: 1647014 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Alternative translocation of protons and halide ions by bacteriorhodopsin.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 11 ·1991-06-01 ·Pages 4751-5

Dér A, Száraz S, Tóth-Boconádi R, Tokaji Z, Keszthelyi L, Stoeckenius W

Abstract

Bacteriorhodopsin (bR568) in purple membrane near pH 2 shifts its absorption maximum from 568 to 605 nm forming the blue protein bRacid605, which no longer transports protons and which shows no transient deprotonation of the Schiff base upon illumination. Continued acid titration with HCl or HBr but not H2SO4 restores the purple chromophore to yield bRHCl564 or bRHBr568. These acid purple forms also regain transmembrane charge transport, but no transient Schiff base deprotonation is observed. In contrast to bR568, no rate decrease of the bRacidpurple transport kinetics is detected in 2H2O; however, the transport rate decreases by a factor of approximately 2 in bRHBr568 compared with bRHCl564. The data indicate that in the acid purple form bR transports the halide anions instead of protons. We present a testable model for the transport mechanism, which should also be applicable to halorhodopsin.

MeSH Terms
Anions Bacteriorhodopsins/metabolism Halobacterium/metabolism Hydrogen-Ion Concentration Kinetics Lasers Light Models, Chemical Protons Schiff Bases Spectrophotometry
Chemicals
Anions Protons Schiff Bases Bacteriorhodopsins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Dér A
Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143.
Száraz S
Tóth-Boconádi R
Tokaji Z
Keszthelyi L
Stoeckenius W
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.11.4751
Published
1991-06-01
Pages
4751-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC51744
Subset
IM
Grants
NIGMS NIH HHS · GM27057 · United States
External Links
PubMed source DOI: 10.1073/pnas.88.11.4751 PMC Full Text View journal details
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