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PMID: 16481433 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Glutamate receptor exocytosis and spine enlargement during chemically induced long-term potentiation.

The Journal of neuroscience : the official journal of the Society for Neuroscience ·Vol. 26 ·No. 7 ·2006-02-15 ·Pages 2000-9

Kopec CD, Li B, Wei W, Boehm J, Malinow R

Abstract

The changes in synaptic morphology and receptor content that underlie neural plasticity are poorly understood. Here, we use a pH-sensitive green fluorescent protein to tag recombinant glutamate receptors and monitor their dynamics onto dendritic spine surfaces. We show that chemically induced long-term potentiation (chemLTP) drives robust exocytosis of AMPA receptors. In contrast, the same stimulus produces a small reduction of NMDA receptors from the spine surface. chemLTP produces similar modification of small and large spines. Interestingly, during chemLTP induction, spines increase in volume before accumulation of AMPA receptors on their surface, indicating that distinct mechanisms underlie changes in morphology and receptor content.

MeSH Terms
Animals Exocytosis/physiology Hippocampus/physiology In Vitro Techniques Long-Term Potentiation/drug effects,physiology Rats Receptors, AMPA/physiology Receptors, Glutamate/physiology Spine/physiology Synapses/drug effects,physiology Transfection
Chemicals
Receptors, AMPA Receptors, Glutamate
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Kopec Charles D
Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA.
Li Bo
Wei Wei
Boehm Jannic
Malinow Roberto
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Article Info
Journal
The Journal of neuroscience : the official journal of the Society for Neuroscience
Abbr.
J Neurosci
ISSN
1529-2401
DOI
10.1523/JNEUROSCI.3918-05.2006
Published
2006-02-15
Pages
2000-9
Language
English
Region
United States
NLM ID
8102140
PMCID
PMC6674938
Subset
IM
External Links
PubMed source DOI: 10.1523/JNEUROSCI.3918-05.2006 PMC Full Text View journal details
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