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PMID: 16489184 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Interacting endogenous and exogenous RNAi pathways in Caenorhabditis elegans.

RNA (New York, N.Y.) ·Vol. 12 ·No. 4 ·2006-04-00 ·Pages 589-97

Lee RC, Hammell CM, Ambros V

Abstract

C. elegans contains numerous small RNAs of ~21-24 nt in length. The microRNAs (miRNAs) are small noncoding RNAs produced by DCR-1- and ALG-dependent processing of self-complementary hairpin transcripts. Endogenous small interfering RNAs (endo-siRNAs), associated with ongoing silencing of protein-coding genes in normal worms, are produced by mechanisms that involve DCR-1 but, unlike miRNAs, also involve RDE-2, RDE-3, RDE-4, RRF-1, and RRF-3. The tiny noncoding (tncRNAs) are similar to endo-siRNAs in their biogenesis except that they are derived from noncoding sequences. These endo-siRNA- and tncRNA-based endogenous RNAi pathways involve some components, including DCR-1 and RDE-4, that are shared with exogenous RNAi, and some components, including RRF-3 and ERI-1, that are specific to endogenous RNAi. rrf-3 and eri-1 mutants are enhanced for some silencing processes and defective for others, suggesting cross-regulatory interactions between RNAi pathways in C. elegans. Microarray expression profiling of RNAi-defective mutant worms further suggests diverse endogenous RNAi pathways for silencing different sets of genes.

MeSH Terms
Animals Base Sequence Blotting, Northern Caenorhabditis elegans/genetics Gene Silencing Mutation Oligonucleotide Probes RNA Interference
Chemicals
Oligonucleotide Probes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lee Rosalind C
Dartmouth Medical School, Department of Genetics, Hanover, New Hamphsire 03755, USA.
Hammell Christopher M
Ambros Victor
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Article Info
Journal
RNA (New York, N.Y.)
Abbr.
RNA
ISSN
1355-8382
DOI
10.1261/rna.2231506
Published
2006-04-00
Epub
2006-00-17
Pages
589-97
Language
English
Region
United States
NLM ID
9509184
PMCID
PMC1421084
Subset
IM
Grants
NIGMS NIH HHS · F32 GM069186 · United States
NIGMS NIH HHS · R01 GM034028 · United States
NIGMS NIH HHS · F32GM69186-1 · United States
NIGMS NIH HHS · GM34028 · United States
External Links
PubMed source DOI: 10.1261/rna.2231506 PMC Full Text View journal details
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