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PMID: 1650188 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Raf-1 is a potential substrate for mitogen-activated protein kinase in vivo.

The Biochemical journal ·Vol. 277 ( Pt 2) ·1991-07-15 ·Pages 573-6

Anderson NG, Li P, Marsden LA, Williams N, Roberts TM, Sturgill TW

Abstract

MAP (mitogen-activated protein) kinase is shown to phosphorylate baculovirally expressed Raf-1 in vitro, generating one major tryptic phosphopeptide which co-migrated with a peptide from Raf-1 32P-labelled in situ. This peptide also undergoes an insulin-dependent increase in labelling. Thus the serine/threonine kinase Raf-1 may be a substrate for MAP kinase in vivo.

MeSH Terms
Animals Calcium-Calmodulin-Dependent Protein Kinases Cell Line Homeodomain Proteins Insecta Peptide Mapping Phosphopeptides/analysis Phosphorylation Protein Kinases/metabolism Proteins/genetics,metabolism Rats Recombinant Proteins/metabolism Thymoma Thymus Neoplasms Transfection
Chemicals
Homeodomain Proteins Phosphopeptides Proteins Recombinant Proteins RAG-1 protein Protein Kinases Calcium-Calmodulin-Dependent Protein Kinases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Anderson N G
Department of Internal Medicine, University of Virginia, Charlottesville 22908.
Li P
Marsden L A
Williams N
Roberts T M
Sturgill T W
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21 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2770573
Published
1991-07-15
Pages
573-6
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1151274
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2770573 PMC Full Text View journal details
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