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PMID: 16507139 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Systematic profiling of cellular phenotypes with spotted cell microarrays reveals mating-pheromone response genes.

Genome biology ·Vol. 7 ·No. 1 ·2006-00-00 ·Pages R6

Narayanaswamy R, Niu W, Scouras AD, Hart GT, Davies J, Ellington AD, Iyer VR, Marcotte EM

Abstract

We have developed spotted cell microarrays for measuring cellular phenotypes on a large scale. Collections of cells are printed, stained for subcellular features, then imaged via automated, high-throughput microscopy, allowing systematic phenotypic characterization. We used this technology to identify genes involved in the response of yeast to mating pheromone. Besides morphology assays, cell microarrays should be valuable for high-throughput in situ hybridization and immunoassays, enabling new classes of genetic assays based on cell imaging.

MeSH Terms
Gene Expression Profiling Genes, Fungal/genetics Genome, Fungal Oligonucleotide Array Sequence Analysis Phenotype Pheromones/genetics Receptors, Mating Factor/genetics Reproducibility of Results Saccharomyces cerevisiae/cytology,genetics Saccharomyces cerevisiae Proteins/genetics
Chemicals
Pheromones Receptors, Mating Factor Saccharomyces cerevisiae Proteins
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Narayanaswamy Rammohan
Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, 2500 Speedway, University of Texas, Austin, TX 78712, USA.
Niu Wei
Scouras Alexander D
Hart G Traver
Davies Jonathan
Ellington Andrew D
Iyer Vishwanath R
Marcotte Edward M
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Article Info
Journal
Genome biology
Abbr.
Genome Biol
ISSN
1474-760X
DOI
10.1186/gb-2006-7-1-r6
Published
2006-00-00
Epub
2006-00-31
Pages
R6
Language
English
Region
England
NLM ID
100960660
PMCID
PMC1431703
Subset
IM
External Links
PubMed source DOI: 10.1186/gb-2006-7-1-r6 PMC Full Text View journal details
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