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PMID: 16552079 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Posttranslational regulation of Mycobacterium tuberculosis extracytoplasmic-function sigma factor sigma L and roles in virulence and in global regulation of gene expression.

Infection and immunity ·Vol. 74 ·No. 4 ·2006-04-00 ·Pages 2457-61

Dainese E, Rodrigue S, Delogu G, Provvedi R, Laflamme L, Brzezinski R, Fadda G, Smith I, Gaudreau L, Palù G, Manganelli R

Abstract

In this report, we demonstrate that SigL is posttranslationally regulated by a specific anti-sigma factor, RslA, and contributes to the expression of at least 28 genes. Several of these genes could mediate important cell envelope-related processes. Importantly, a sigL-rslA mutant strain was significantly attenuated in a mouse model of infection.

MeSH Terms
Animals Base Sequence Gene Expression Regulation, Bacterial/immunology Mice Mice, Inbred BALB C Mice, Inbred C57BL Mice, Inbred DBA Molecular Sequence Data Mycobacterium tuberculosis/genetics,pathogenicity Protein Processing, Post-Translational/immunology Sigma Factor/biosynthesis,genetics,physiology Tuberculosis, Pulmonary/microbiology,mortality Virulence Factors/biosynthesis,genetics,physiology
Chemicals
Sigma Factor Virulence Factors
Authors & Affiliations
11 authors, click to expand affiliations / ORCID
Dainese Elisa
Department of Histology, Microbiology and Medical Biotechnologies, University of Padova, Via Gabelli 63, 35100 Padova, Italy.
Rodrigue Sébastien
Delogu Giovanni
Provvedi Roberta
Laflamme Liette
Brzezinski Ryszard
Fadda Giovanni
Smith Issar
Gaudreau Luc
Palù Giorgio
Manganelli Riccardo
References (28)
28 references, click to expand
  1. RshA, an anti-sigma factor that regulates the activity of the mycobacterial stress response sigma factor SigH.
    Mol Microbiol. 2003 Nov;50(3):949-59 PMID: 14617153
  2. Multiple sigma subunits and the partitioning of bacterial transcription space.
    Annu Rev Microbiol. 2003;57:441-66 PMID: 14527287
  3. Attenuation of late-stage disease in mice infected by the Mycobacterium tuberculosis mutant lacking the SigF alternate sigma factor and identification of SigF-dependent genes by microarray analysis.
    Infect Immun. 2004 Mar;72(3):1733-45 PMID: 14977982
  4. The principal sigma factor sigA mediates enhanced growth of Mycobacterium tuberculosis in vivo.
    Mol Microbiol. 2004 Mar;51(6):1551-62 PMID: 15009884
  5. Mycobacterium tuberculosis ECF sigma factor sigC is required for lethality in mice and for the conditional expression of a defined gene set.
    Mol Microbiol. 2004 Apr;52(1):25-38 PMID: 15049808
  6. The extra cytoplasmic function sigma factor sigma(E) is essential for Mycobacterium tuberculosis virulence in mice.
    Infect Immun. 2004 May;72(5):3038-41 PMID: 15102817
  7. Transcription regulation by the Mycobacterium tuberculosis alternative sigma factor SigD and its role in virulence.
    J Bacteriol. 2004 Oct;186(19):6605-16 PMID: 15375142
  8. Protection to mice afforded by BCG vaccines against an aerogenic challenge by three mycobacteria of decreasing virulence.
    Tubercle. 1985 Dec;66(4):267-76 PMID: 3936247
  9. DNA sequence, structure and gene expression of mycobacteriophage L5: a phage system for mycobacterial genetics.
    Mol Microbiol. 1993 Feb;7(3):395-405 PMID: 8459766
  10. Gene knockout reveals a novel gene cluster for the synthesis of a class of cell wall lipids unique to pathogenic mycobacteria.
    J Biol Chem. 1997 Jul 4;272(27):16741-5 PMID: 9201977
  11. Differential expression of 10 sigma factor genes in Mycobacterium tuberculosis.
    Mol Microbiol. 1999 Jan;31(2):715-24 PMID: 10027986
  12. A protein secretion pathway critical for Mycobacterium tuberculosis virulence is conserved and functional in Mycobacterium smegmatis.
    J Bacteriol. 2005 Feb;187(4):1238-45 PMID: 15687187
  13. Gap, a mycobacterial specific integral membrane protein, is required for glycolipid transport to the cell surface.
    Mol Microbiol. 2005 Oct;58(2):426-40 PMID: 16194230
  14. The Mycobacterium tuberculosis extracytoplasmic-function sigma factor SigL regulates polyketide synthases and secreted or membrane proteins and is required for virulence.
    J Bacteriol. 2005 Oct;187(20):7062-71 PMID: 16199577
  15. A recombinant Mycobacterium tuberculosis in vitro transcription system.
    FEMS Microbiol Lett. 2006 Feb;255(1):140-7 PMID: 16436073
  16. Significance analysis of microarrays applied to the ionizing radiation response.
    Proc Natl Acad Sci U S A. 2001 Apr 24;98(9):5116-21 PMID: 11309499
  17. Analysis of the phthiocerol dimycocerosate locus of Mycobacterium tuberculosis. Evidence that this lipid is involved in the cell wall permeability barrier.
    J Biol Chem. 2001 Jun 8;276(23):19845-54 PMID: 11279114
  18. The Mycobacterium tuberculosis ECF sigma factor sigmaE: role in global gene expression and survival in macrophages.
    Mol Microbiol. 2001 Jul;41(2):423-37 PMID: 11489128
  19. The alternative sigma factor SigH regulates major components of oxidative and heat stress responses in Mycobacterium tuberculosis.
    J Bacteriol. 2001 Oct;183(20):6119-25 PMID: 11567012
  20. Evaluation of a nutrient starvation model of Mycobacterium tuberculosis persistence by gene and protein expression profiling.
    Mol Microbiol. 2002 Feb;43(3):717-31 PMID: 11929527
  21. Role of the extracytoplasmic-function sigma factor sigma(H) in Mycobacterium tuberculosis global gene expression.
    Mol Microbiol. 2002 Jul;45(2):365-74 PMID: 12123450
  22. Novel Mycobacterium tuberculosis anti-sigma factor antagonists control sigmaF activity by distinct mechanisms.
    Mol Microbiol. 2002 Sep;45(6):1527-40 PMID: 12354223
  23. Specialized transduction: an efficient method for generating marked and unmarked targeted gene disruptions in Mycobacterium tuberculosis, M. bovis BCG and M. smegmatis.
    Microbiology. 2002 Oct;148(Pt 10):3007-17 PMID: 12368434
  24. Attenuation of Mycobacterium tuberculosis by disruption of a mas-like gene or a chalcone synthase-like gene, which causes deficiency in dimycocerosyl phthiocerol synthesis.
    J Bacteriol. 2003 May;185(10):2999-3008 PMID: 12730158
  25. Virulence attenuation of two Mas-like polyketide synthase mutants of Mycobacterium tuberculosis.
    Microbiology. 2003 Jul;149(Pt 7):1837-47 PMID: 12855735
  26. Mycobacterium tuberculosis pathogenesis and molecular determinants of virulence.
    Clin Microbiol Rev. 2003 Jul;16(3):463-96 PMID: 12857778
  27. Biochemical function of msl5 (pks8 plus pks17) in Mycobacterium tuberculosis H37Rv: biosynthesis of monomethyl branched unsaturated fatty acids.
    J Bacteriol. 2003 Aug;185(15):4620-5 PMID: 12867474
  28. Sigma factors and global gene regulation in Mycobacterium tuberculosis.
    J Bacteriol. 2004 Feb;186(4):895-902 PMID: 14761983
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/IAI.74.4.2457-2461.2006
Published
2006-04-00
Pages
2457-61
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC1418919
Subset
IM
Grants
NIAID NIH HHS · R01 AI044856 · United States
NIAID NIH HHS · R56 AI044856 · United States
NIAID NIH HHS · AI-44856 · United States
External Links
PubMed source DOI: 10.1128/IAI.74.4.2457-2461.2006 PMC Full Text View journal details
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