Plaque Formation by Rickettsia conori in WI-38, DBS-FRhL-2, L-929, HeLa, and Chicken Embryo Cells.

Infection and immunity ·Vol. 10 ·No. 5 ·1974-11-00 ·Pages 1152-5

Osterman JV, Parr RP

Abstract

Mammalian cells particularly suitable for the study of specialized aspects of rickettsial biology were tested for their ability to support plaque formation by Rickettsia conori. The detection of plaques was substantially influenced by the combination of growth medium and cell type used. Large plaques (2.0 to 3.0 mm in diameter) occurred by 8 days postinfection in WI-38 and DBS-FRhL-2 cells supported by medium 199. Smaller plaques (0.5 to 1.0 mm in diameter) were seen in L-929 and HeLa cells at 8 to 11 days postinfection and were more discernible in cells supported with Eagle minimal essential medium. Chicken embryo cells maintained in Dulbecco's modified Eagle medium exhibited large spherical plaques with a diameter of approximately 1.5 mm by 8 days postinfection.

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Osterman J V

Department of Hazardous Microorganisms, Walter Reed Army Institute of Research, Washington, D.C. 20012.

Parr R P

References (12)

12 references, click to expand

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Article Info

Journal

Infection and immunity

Abbr.

Infect Immun

ISSN

0019-9567

DOI

10.1128/iai.10.5.1152-1155.1974

Published

1974-11-00

Pages

1152-5

Language

English

Region

United States

NLM ID

0246127

PMCID

PMC423075

External Links

PubMed source DOI: 10.1128/iai.10.5.1152-1155.1974 PMC Full Text View journal details

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Plaque Formation by Rickettsia conori in WI-38, DBS-FRhL-2, L-929, HeLa, and Chicken Embryo Cells.

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