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PMID: 16581831 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Characterization of two oxidatively modified phospholipids in mixed monolayers with DPPC.

Biophysical journal ·Vol. 90 ·No. 12 ·2006-06-15 ·Pages 4488-99

Sabatini K, Mattila JP, Megli FM, Kinnunen PK

Abstract

The properties of two oxidatively modified phospholipids viz. 1-palmitoyl-2-(9'-oxo-nonanoyl)-sn-glycero-3-phosphocholine (PoxnoPC) and 1-palmitoyl-2-azelaoyl-sn-glycero-3-phosphocholine (PazePC), were investigated using a Langmuir balance, recording force-area (pi-A) isotherms and surface potential psi. In mixed monolayers with 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) a progressive disappearance of the liquid expanded-liquid condensed transition and film expansion was observed with increasing content of the oxidized phospholipids. The above is in agreement with fluorescence microscopy of the monolayers, which revealed an increase in the liquid expanded region of DPPC monolayers. At a critical pressure pi(s) approximately 42 mN/m both Poxo- and PazePC induced a deflection in the pi-A isotherms, which could be rationalized in terms of reorientation of the oxidatively modified acyl chains into aqueous phase (adaptation of the so-called extended conformation), followed upon further film compression by solubilization of the oxidized phospholipids into the aqueous phase. Surface potential displayed a discontinuity at the same value of area/molecule, corresponding to the loss of the oxidized phospholipids from the monolayers. Our data support the view that lipid oxidation modifies both the small-scale structural dynamics of biological membranes as well as their more macroscopic lateral organization. Accordingly, oxidatively modified lipids can be expected to influence the organization and functions of membrane associated proteins.

MeSH Terms
1,2-Dipalmitoylphosphatidylcholine/chemistry Complex Mixtures/chemistry Lipid Bilayers/chemistry Lipid Peroxidation Liposomes/chemistry Membrane Fluidity Molecular Conformation Oxidation-Reduction Oxygen/chemistry Phospholipids/chemistry Solubility Structure-Activity Relationship
Chemicals
Complex Mixtures Lipid Bilayers Liposomes Phospholipids 1,2-Dipalmitoylphosphatidylcholine Oxygen
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Sabatini Karen
Dipartimento di Biochimica e Biologia Molecolare, Università di Bari, Centro di Studio sui Mitocondri e Metabolismo Energetico--CNR, Bari, Italy.
Mattila Juha-Pekka
Megli Francesco M
Kinnunen Paavo K J
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Article Info
Journal
Biophysical journal
Abbr.
Biophys J
ISSN
0006-3495
DOI
10.1529/biophysj.105.080176
Published
2006-06-15
Epub
2006-00-31
Pages
4488-99
Language
English
Region
United States
NLM ID
0370626
PMCID
PMC1471873
Subset
IM
External Links
PubMed source DOI: 10.1529/biophysj.105.080176 PMC Full Text View journal details
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