Home LiteratureArticle Details
PMID: 16593326 Published · ppublish English Journal Article

A short 5' flanking region containing conserved sequences is required for silkworm alanine tRNA gene activity.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 11 ·1983-06-00 ·Pages 3416-20

Larson D, Bradford-Wilcox J, Young LS, Sprague KU

Abstract

Using partially deleted genes, we have identified an upstream control signal required for transcription of a Bombyx mori (silkworm) tRNA(2) (Ala) gene. The 5' boundary of this essential region lies between 34 and 11 nucleotides preceding the transcription initiation site. Sequences in the 5' half of the tRNA(2) (Ala) coding region are also important. Both regions contain oligonucleotides that are conserved among several Bombyx genes transcribed by RNA polymerase III, suggesting that these sequences may have a general control function.

Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Larson D
Institute of Molecular Biology and Department of Biology, University of Oregon, Eugene, Oregon 97403.
Bradford-Wilcox J
Young L S
Sprague K U
References (23)
23 references, click to expand
  1. Extracellular nucleases of pseudomonas BAL 31. III. Use of the double-strand deoxyriboexonuclease activity as the basis of a convenient method for the mapping of fragments of DNA produced by cleavage with restriction enzymes.
    Nucleic Acids Res. 1978 May;5(5):1445-64 PMID: 307230
  2. The primary transcription product of a silkworm alanine tRNA gene: identification of in vitro sites of initiation, termination and processing.
    Cell. 1979 Dec;18(4):1217-29 PMID: 519766
  3. A quantitative assay for Xenopus 5S RNA gene transcription in vitro.
    Cell. 1981 Jun;24(3):809-17 PMID: 6166388
  4. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  5. Analysis of transcription of the human Alu family ubiquitous repeating element by eukaryotic RNA polymerase III.
    Nucleic Acids Res. 1981 Dec 11;9(23):6439-56 PMID: 6275362
  6. sigma, a repetitive element found adjacent to tRNA genes of yeast.
    Proc Natl Acad Sci U S A. 1982 Jul;79(13):4138-42 PMID: 6287468
  7. An in vitro RNA polymerase III system from S. cerevisiae: effects of deletions and point mutations upon SUP4 gene transcription.
    Nucleic Acids Res. 1982 Dec 20;10(24):8127-43 PMID: 6298710
  8. Identification of regulatory sequences contained in the 5'-flanking region of Drosophila lysine tRNA2 genes.
    J Biol Chem. 1981 Dec 10;256(23):12424-9 PMID: 6913581
  9. The minimum intragenic sequences required for promotion of eukaryotic tRNA gene transcription.
    Nucleic Acids Res. 1982 Sep 25;10(18):5393-406 PMID: 6924209
  10. The 5- flanking sequences of Drosophila tRNAArg genes control their in vitro transcription in a Drosophila cell extract.
    J Biol Chem. 1982 Dec 25;257(24):14738-44 PMID: 6924656
  11. Control region for adenovirus VA RNA transcription.
    Proc Natl Acad Sci U S A. 1981 Jun;78(6):3378-82 PMID: 6943546
  12. Internal control regions for transcription of eukaryotic tRNA genes.
    Proc Natl Acad Sci U S A. 1981 Nov;78(11):6657-61 PMID: 6947245
  13. Promoter of a eukaryotic tRNAPro gene is composed of three noncontiguous regions.
    Proc Natl Acad Sci U S A. 1982 Feb;79(4):1195-9 PMID: 6951168
  14. Mutations of the yeast SUP4 tRNATyr locus: transcription of the mutant genes in vitro.
    Cell. 1980 Nov;22(2 Pt 2):415-25 PMID: 7004643
  15. Transcription initiation of eucaryotic transfer RNA genes.
    Cell. 1982 May;29(1):3-5 PMID: 7049399
  16. Enhancing elements for activation of eukaryotic promoters.
    Nature. 1982 May 6;297(5861):17-8 PMID: 7070530
  17. A split promoter for a eucaryotic tRNA gene.
    Cell. 1981 May;24(2):573-85 PMID: 7237560
  18. Two conserved sequence blocks within eukaryotic tRNA genes are major promoter elements.
    Nature. 1981 Dec 17;294(5842):626-31 PMID: 7312050
  19. A control region in the center of the 5S RNA gene directs specific initiation of transcription: I. The 5' border of the region.
    Cell. 1980 Jan;19(1):13-25 PMID: 7357599
  20. A control region in the center of the 5S RNA gene directs specific initiation of transcription: II. The 3' border of the region.
    Cell. 1980 Jan;19(1):27-35 PMID: 7357604
  21. 5' flanking sequence signals are required for activity of silkworm alanine tRNA genes in homologous in vitro transcription systems.
    Cell. 1980 Nov;22(1 Pt 1):171-8 PMID: 7428038
  22. Transcriptional control regions of the adenovirus VAI RNA gene.
    Cell. 1980 Nov;22(2 Pt 2):405-13 PMID: 7448868
  23. Silkworm 5S RNA and alanine tRNA genes share highly conserved 5' flanking and coding sequences.
    Mol Cell Biol. 1982 Dec;2(12):1524-31 PMID: 14582194
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.11.3416
Published
1983-06-00
Pages
3416-20
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC394054
External Links
PubMed source DOI: 10.1073/pnas.80.11.3416 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2