Nonsense mutations in the Chlamydomonas chloroplast gene that codes for the large subunit of ribulosebisphosphate carboxylase/oxygenase.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 16 ·1985-08-00 ·Pages 5460-4

Spreitzer RJ, Goldschmidt-Clermont M, Rahire M, Rochaix JD

Abstract

The Chlamydomonas reinhardtii chloroplast mutants 18-5B and 18-7G lack both the chloroplast-encoded large subunit and nuclear-encoded small subunit of the chloroplast enzyme ribulose-1,5-bisphosphate carboxylase/oxygenase (EC 4.1.1.39). A chloroplast intergenic-suppression model has been postulated to account for the genetic instability of 18-5B revertants. Here, we have determined the molecular basis of the 18-5B and 18-7G mutants. They contain nonsense mutations close to the 3' and 5' ends of their large-subunit genes, respectively. Pulse-chase experiments revealed that the 18-5B mutant produces a truncated large subunit that is unstable. In connection with previous experiments, this work identifies nonsense suppression in the chloroplast. Small subunits are also synthesized but then degraded in the mutants. Thus, the coordinated absence of subunits is achieved through degradation of the small subunit in the specific absence of the large subunit.

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Spreitzer R J

Department of Molecular Biology, University of Geneva, 1211 Geneva 4, Switzerland.

Goldschmidt-Clermont M

Rahire M

Rochaix J D

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.82.16.5460

Published

1985-08-00

Pages

5460-4

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC390589

External Links

PubMed source DOI: 10.1073/pnas.82.16.5460 PMC Full Text View journal details

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Nonsense mutations in the Chlamydomonas chloroplast gene that codes for the large subunit of ribulosebisphosphate carboxylase/oxygenase.

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