Home LiteratureArticle Details
PMID: 16594033 Published · ppublish English Journal Article

Dual promoter of Agrobacterium tumefaciens mannopine synthase genes is regulated by plant growth hormones.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 9 ·1989-05-00 ·Pages 3219-23

Langridge WH, Fitzgerald KJ, Koncz C, Schell J, Szalay AA

Abstract

Temporal and spacial distribution of mannopine synthase (mas) promoter activity was determined throughout the development of transgenic tobacco plants using bacterial luciferase luxA and luxB as reporter genes. Luciferase activity was determined by luminometry in vitro and visualized by computer-enhanced single-photon video imaging in vivo. The activity of the mas dual promoters increased basipetally in developing plants and was wound-inducible in leaf and stem tissue. Hormone bioassays with isolated plant tissues and tumors deficient in the transferred DNA (T-DNA)-encoded genes iaaM, iaaH, and ipt indicated that activity of the mas dual promoters is regulated by auxin and enhanced by cytokinin in both differentiated and tumorous plant cells.

Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Langridge W H
University of Alberta, Plant Molecular Genetics and Plant Biotechnology Centre and Department of Cell Biology, Medical Sciences Building, Edmonton, AB T6G 2P5, Canada.
Fitzgerald K J
Koncz C
Schell J
Szalay A A
References (18)
18 references, click to expand
  1. Identification of an essential upstream element in the nopaline synthase promoter by stable and transient assays.
    Proc Natl Acad Sci U S A. 1987 Aug;84(16):5745-9 PMID: 16593869
  2. Inhibition of polar auxin transport by ethylene.
    Plant Physiol. 1967 Sep;42(9):1224-8 PMID: 6076113
  3. Isolation of a dual plant promoter fragment from the Ti plasmid of Agrobacterium tumefaciens.
    EMBO J. 1984 Dec 1;3(12):2723-30 PMID: 16453574
  4. Genetic identification of functions of TR-DNA transcripts in octopine crown galls.
    EMBO J. 1984 Jan;3(1):141-6 PMID: 16453491
  5. The ocs element: a 16 base pair palindrome essential for activity of the octopine synthase enhancer.
    EMBO J. 1987 Nov;6(11):3203-8 PMID: 16453801
  6. A simple and general method for transferring genes into plants.
    Science. 1985 Mar 8;227(4691):1229-31 PMID: 17757866
  7. Transgenic plants as tools to study the molecular organization of plant genes.
    Science. 1987 Sep 4;237(4819):1176-83 PMID: 17801640
  8. T-DNA of Agrobacterium tumefaciens encodes an enzyme of cytokinin biosynthesis.
    Proc Natl Acad Sci U S A. 1984 Oct;81(19):5994-8 PMID: 6091129
  9. Size, location and polarity of T-DNA-encoded transcripts in nopaline crown gall tumors; common transcripts in octopine and nopaline tumors.
    Cell. 1983 Apr;32(4):1045-56 PMID: 6301679
  10. The complete nucleotide sequence of the TL-DNA of the Agrobacterium tumefaciens plasmid pTiAch5.
    EMBO J. 1984 Apr;3(4):835-46 PMID: 6327292
  11. Genetic analysis of crown gall: fine structure map of the T-DNA by site-directed mutagenesis.
    Cell. 1981 Nov;27(1 Pt 2):143-53 PMID: 6276020
  12. The T-region of Ti plasmids codes for an enzyme synthesizing indole-3-acetic acid.
    Eur J Biochem. 1984 Jan 16;138(2):387-91 PMID: 6365544
  13. [Preparation and properties of new catabolic substrates for two types of oncogenic plasmids of Agrobacterium tumefaciens].
    C R Seances Acad Sci D. 1980 May 5;290(17):1173-6 PMID: 6772324
  14. Nopaline synthase: transcript mapping and DNA sequence.
    J Mol Appl Genet. 1982;1(6):561-73 PMID: 7153689
  15. Nucleotide sequence and transcript map of the Agrobacterium tumefaciens Ti plasmid-encoded octopine synthase gene.
    J Mol Appl Genet. 1982;1(6):499-511 PMID: 7153687
  16. Streptomycin-resistant plants from callus culture of haploid tobacco.
    Nat New Biol. 1973 Jul 4;244(131):29-30 PMID: 4515911
  17. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
  18. Expression and assembly of functional bacterial luciferase in plants.
    Proc Natl Acad Sci U S A. 1987 Jan;84(1):131-5 PMID: 16593793
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.9.3219
Published
1989-05-00
Pages
3219-23
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC287101
External Links
PubMed source DOI: 10.1073/pnas.86.9.3219 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2