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PMID: 6091129 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

T-DNA of Agrobacterium tumefaciens encodes an enzyme of cytokinin biosynthesis.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 19 ·1984-10-00 ·Pages 5994-8

Akiyoshi DE, Klee H, Amasino RM, Nester EW, Gordon MP

Abstract

Phytohormone overproduction in crown gall tumors is due to the expression of several T-DNA genes. The data strongly suggest that the tmr gene (transcript 4) is responsible for cytokinin overproduction by encoding dimethylallyl-pyrophosphate:AMP dimethylallyltransferase (DMA transferase), an enzyme directly involved in cytokinin biosynthesis. Cell-free extracts of Escherichia coli strains containing the tmr gene from pTiA6NC had DMA transferase activity. No activity was present in the control strain containing only the plasmid vector. The cytokinins synthesized were isopentenyladenine, isopentenyladenosine, and isopentenyladenosine 5'-monophosphate. DMA transferase activity was also detected in cloned crown gall tumors incited by Agrobacterium tumefaciens wild-type A6NC and a tms mutant. Enzymatic activity in cell-free extracts of E. coli and tumors could be abolished by transposon insertion within the tmr gene.

MeSH Terms

Base Sequence Cytokinins/genetics DNA Restriction Enzymes DNA, Bacterial/genetics Dimethylallyltranstransferase/genetics Genes Genes, Bacterial Plant Growth Regulators/genetics Plant Tumors/microbiology Plasmids Rhizobium/enzymology,genetics Transcription, Genetic Transferases/genetics

Chemicals

Cytokinins DNA, Bacterial Plant Growth Regulators T-DNA Transferases Dimethylallyltranstransferase DNA Restriction Enzymes

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Akiyoshi D E

Klee H

Amasino R M

Nester E W

Gordon M P

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24 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.81.19.5994

Published

1984-10-00

Pages

5994-8

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC391845

Subset

Grants

NCI NIH HHS · R0I CA12015-12 · United States

NIGMS NIH HHS · T32 GM07187 · United States

External Links

PubMed source DOI: 10.1073/pnas.81.19.5994 PMC Full Text View journal details

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T-DNA of Agrobacterium tumefaciens encodes an enzyme of cytokinin biosynthesis.

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