Home LiteratureArticle Details
PMID: 6366736 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

The sequence of the tms transcript 2 locus of the A. tumefaciens plasmid pTiA6 and characterization of the mutation in pTiA66 that is responsible for auxin attenuation.

Nucleic acids research ·Vol. 12 ·No. 3 ·1984-02-10 ·Pages 1447-61

Sciaky D, Thomashow MF

Abstract

The incorporation of Ti plasmid sequences, the T-DNA, into the genomes of dicotyledenous plants causes the formation of tumors. Here we report the nucleotide sequence of one of the T-DNA "oncogenes", the transcript 2 gene of pTiA6 and we further characterize the 2.7 Kb element that has spontaneously inserted into this gene in plasmid pTiA66. The results indicate that the transcript 2 portion of the T-DNA has an open reading frame that could encode a polypeptide of 49.8 Kd. The open reading frame is surrounded by sequences that typically have roles in eucaryotic gene expression. Nucleotide sequence and Southern blot analysis also indicates that the 2.7 Kb insert in the transcript 2 gene of pTiA66 is located within the coding sequence of the gene and suggests that the element is an insertion sequence. We designate this element, IS66.

MeSH Terms
Amino Acid Sequence Base Sequence Coliphages/genetics Escherichia coli/genetics Genes Genes, Bacterial Indoleacetic Acids/metabolism Mutation Nucleic Acid Conformation Nucleic Acid Hybridization Plant Growth Regulators/metabolism Plant Tumors/microbiology Plants/microbiology Plasmids Rhizobium/genetics Transcription, Genetic
Chemicals
Indoleacetic Acids Plant Growth Regulators
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sciaky D
Thomashow M F
References (43)
43 references, click to expand
  1. Regulatory sequences involved in the promotion and termination of RNA transcription.
    Annu Rev Genet. 1979;13:319-53 PMID: 94251
  2. Relationship between the limited and wide host range octopine-type Ti plasmids of Agrobacterium tumefaciens.
    J Bacteriol. 1981 May;146(2):484-93 PMID: 6260751
  3. Size, location and polarity of T-DNA-encoded transcripts in nopaline crown gall tumors; common transcripts in octopine and nopaline tumors.
    Cell. 1983 Apr;32(4):1045-56 PMID: 6301679
  4. Further procedures for sequence analysis by computer.
    Nucleic Acids Res. 1978 Mar;5(3):1013-6 PMID: 643613
  5. Recombination between higher plant DNA and the Ti plasmid of Agrobacterium tumefaciens.
    Proc Natl Acad Sci U S A. 1980 Nov;77(11):6448-52 PMID: 16592915
  6. Nucleotide sequence and transcript map of the Agrobacterium tumefaciens Ti plasmid-encoded octopine synthase gene.
    J Mol Appl Genet. 1982;1(6):499-511 PMID: 7153687
  7. The conserved part of the T-region in Ti-plasmids expresses four proteins in bacteria.
    EMBO J. 1983;2(3):403-9 PMID: 11894956
  8. A simple method for displaying the hydropathic character of a protein.
    J Mol Biol. 1982 May 5;157(1):105-32 PMID: 7108955
  9. Transcriptional control regions: nucleotide sequence requirements for initiation by RNA polymerase II and III.
    Curr Top Microbiol Immunol. 1981;93:25-46 PMID: 7026181
  10. Separation of signal transduction and adaptation functions of the aspartate receptor in bacterial sensing.
    Science. 1983 Jun 3;220(4601):1016-20 PMID: 6302843
  11. Grown gall plant tumors of abnormal morphology, induced by Agrobacterium tumefaciens carrying mutated octopine Ti plasmids; analysis of T-DNA functions.
    Gene. 1981 Jun-Jul;14(1-2):33-50 PMID: 6266929
  12. Integration and organization of Ti plasmid sequences in crown gall tumors.
    Cell. 1980 Mar;19(3):729-39 PMID: 7363328
  13. A versatile primer for DNA sequencing in the M13mp2 cloning system.
    Gene. 1980 Jun;10(1):69-73 PMID: 6250947
  14. Structure and transcription of the nopaline synthase gene region of T-DNA.
    Nucleic Acids Res. 1983 Jan 25;11(2):369-85 PMID: 6298724
  15. A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments.
    Gene. 1982 Oct;19(3):269-76 PMID: 6295880
  16. Extra DNA in the T region of crown gall Ti-plasmid pTiA66.
    Plasmid. 1983 Sep;10(2):199-203 PMID: 6314413
  17. Agrobacterium tumefaciens mutants affected in crown gall tumorigenesis and octopine catabolism.
    J Bacteriol. 1980 Nov;144(2):732-43 PMID: 6253441
  18. The TL-DNA in octopine crown-gall tumours codes for seven well-defined polyadenylated transcripts.
    EMBO J. 1982;1(1):139-46 PMID: 16453403
  19. Nuclear and polysomal transcripts of T-DNA in octopine crown gall suspension and callus cultures.
    Mol Gen Genet. 1981;182(2):255-62 PMID: 6945473
  20. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  21. Nopaline synthase: transcript mapping and DNA sequence.
    J Mol Appl Genet. 1982;1(6):561-73 PMID: 7153689
  22. Sizes and map positions of several plasmid-DNA-encoded transcripts in octopine-type crown gall tumors.
    Proc Natl Acad Sci U S A. 1982 Jan;79(1):76-80 PMID: 6275392
  23. Stable incorporation of plasmid DNA into higher plant cells: the molecular basis of crown gall tumorigenesis.
    Cell. 1977 Jun;11(2):263-71 PMID: 890735
  24. Functional relationships between transcriptional control signals of the thymidine kinase gene of herpes simplex virus.
    Cell. 1982 Dec;31(2 Pt 1):355-65 PMID: 6297762
  25. The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites.
    Proc Natl Acad Sci U S A. 1974 Apr;71(4):1342-6 PMID: 4598299
  26. Restriction endonuclease mapping of the octopine tumor-inducing plasmid pTiAch5 of Agrobacterium tumefaciens.
    Plasmid. 1981 Sep;6(2):249-53 PMID: 6272339
  27. Genetic analysis of T-DNA transcripts in nopaline crown galls.
    Cell. 1983 Apr;32(4):1057-67 PMID: 6839358
  28. Extensive homology between membrane-associated components of histidine and maltose transport systems of Salmonella typhimurium and Escherichia coli.
    J Biol Chem. 1982 Sep 10;257(17):9915-8 PMID: 7050111
  29. Genetic Identification of functions of TL-DNA transcripts in octopine crown galls.
    EMBO J. 1982;1(1):147-52 PMID: 16453404
  30. Transcription of T-DNA in octopine and nopaline crown gall tumours is inhibited by low concentrations of alpha-amanitin.
    Nucleic Acids Res. 1981 Oct 10;9(19):4801-12 PMID: 6171771
  31. Studies on Certain Physiological Characters of Phytomonas tumefaciens, Phytomonas rhizogenes and Bacillus radiobacter: Part II.
    J Bacteriol. 1934 Dec;28(6):597-618 PMID: 16559769
  32. Promoter sequences of eukaryotic protein-coding genes.
    Science. 1980 Sep 19;209(4463):1406-14 PMID: 6251548
  33. Kilo-sequencing: an ordered strategy for rapid DNA sequence data acquisition.
    Nucleic Acids Res. 1983 Jan 25;11(2):349-68 PMID: 6298723
  34. Computer programs for nucleic acid sequence manipulation.
    Nucleic Acids Res. 1982 Jan 11;10(1):91-101 PMID: 6278414
  35. Sequence data handling by computer.
    Nucleic Acids Res. 1977 Nov;4(11):4037-51 PMID: 593900
  36. Tumor DNA structure in plant cells transformed by A. tumefaciens.
    Science. 1980 Sep 19;209(4463):1385-91 PMID: 6251546
  37. T-DNA from Agrobacterium Ti plasmid is in the nuclear DNA fraction of crown gall tumor cells.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):4060-4 PMID: 16592850
  38. Variation in hormone autonomy and regenerative potential of cells transformed by strain A66 of Agrobacterium tumefaciens.
    Cell. 1982 Dec;31(3 Pt 2):605-12 PMID: 6297775
  39. Identification of sequences involved in the polyadenylation of higher plant nuclear transcripts using Agrobacterium T-DNA genes as models.
    EMBO J. 1983;2(3):419-26 PMID: 11894958
  40. A new method for sequencing DNA.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
  41. 3' non-coding region sequences in eukaryotic messenger RNA.
    Nature. 1976 Sep 16;263(5574):211-4 PMID: 822353
  42. Genetic analysis of crown gall: fine structure map of the T-DNA by site-directed mutagenesis.
    Cell. 1981 Nov;27(1 Pt 2):143-53 PMID: 6276020
  43. Computer programs for the assembly of DNA sequences.
    Nucleic Acids Res. 1979 Sep 25;7(2):529-45 PMID: 493154
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.3.1447
Published
1984-02-10
Pages
1447-61
Language
English
Region
England
NLM ID
0411011
PMCID
PMC318588
Subset
IM
Grants
NCI NIH HHS · CA 31313 · United States
Databases
GENBANK
X00409
External Links
PubMed source DOI: 10.1093/nar/12.3.1447 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2