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PMID: 1660147 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A contingent replication assay for the detection of protein-protein interactions in animal cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 23 ·1991-12-01 ·Pages 10686-90

Vasavada HA, Ganguly S, Germino FJ, Wang ZX, Weissman SM

Abstract

We have developed a sensitive and rapid assay system termed the contingent replication assay (CRA) for selecting cDNAs with desired functional properties from a cDNA library. The system functions in animal cells and permits enrichment of the desired cDNA in small-scale and convenient experiments. The assay can be used for the enrichment of proteins that activate transcription from conditional enhancers, bind to specific DNA sequences, or interact with target proteins of interest. In this communication we report the application of this assay to study protein-protein interactions in animal cells.

MeSH Terms
Animals Antigens, Polyomavirus Transforming/genetics,metabolism Cell Line Chloramphenicol O-Acetyltransferase/genetics,metabolism DNA Replication DNA-Binding Proteins/genetics,metabolism Fungal Proteins/genetics,metabolism Gene Library Genetic Techniques Herpes Simplex Virus Protein Vmw65 Plasmids Proteins/genetics,metabolism Recombinant Fusion Proteins/metabolism Repressor Proteins/genetics Saccharomyces cerevisiae Proteins Simian virus 40/genetics Transcription Factors Transfection Viral Proteins/genetics,metabolism
Chemicals
Antigens, Polyomavirus Transforming DNA-Binding Proteins Fungal Proteins GAL4 protein, S cerevisiae Herpes Simplex Virus Protein Vmw65 Proteins Recombinant Fusion Proteins Repressor Proteins Saccharomyces cerevisiae Proteins Transcription Factors Viral Proteins Chloramphenicol O-Acetyltransferase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Vasavada H A
Department of Genetics, Yale University School of Medicine, New Haven, CT 06510.
Ganguly S
Germino F J
Wang Z X
Weissman S M
References (26)
26 references, click to expand
  1. Cloning of PI3 kinase-associated p85 utilizing a novel method for expression/cloning of target proteins for receptor tyrosine kinases.
    Cell. 1991 Apr 5;65(1):83-90 PMID: 1849461
  2. Use of gene fusions and protein-protein interaction in the isolation of a biologically active regulatory protein: the replication initiator protein of plasmid R6K.
    Proc Natl Acad Sci U S A. 1983 Nov;80(22):6848-52 PMID: 6316329
  3. Cloning of cDNAs for cellular proteins that bind to the retinoblastoma gene product.
    Nature. 1991 Jul 18;352(6332):251-4 PMID: 1857421
  4. A simple and efficient cDNA library subtraction procedure: isolation of human retina-specific cDNA clones.
    Nucleic Acids Res. 1991 Apr 25;19(8):1954 PMID: 2030979
  5. Factors involved in specific transcription by mammalian RNA polymerase II. Purification and functional analysis of general transcription factor IIE.
    J Biol Chem. 1991 May 15;266(14):9304-8 PMID: 2026628
  6. Man-made antibodies.
    Nature. 1991 Jan 24;349(6307):293-9 PMID: 1987490
  7. Factors involved in specific transcription by mammalian RNA polymerase II: identification of general transcription factor TFIIG.
    Proc Natl Acad Sci U S A. 1990 Dec;87(23):9158-62 PMID: 2251257
  8. Resolution and partial characterization of factors required for in vitro transcription by mammalian RNA polymerase II.
    Proc Natl Acad Sci U S A. 1989 Aug;86(16):6106-10 PMID: 2762317
  9. A model system for the rescue of cDNA encoding transacting transcription activator by contingent replication assay.
    Indian J Biochem Biophys. 1988 Dec;25(6):488-94 PMID: 2855627
  10. Contingent replication assay (CRA) procedure for rapid isolation of enhancers.
    Gene. 1987;55(1):29-40 PMID: 3040530
  11. Fos and Jun: the AP-1 connection.
    Cell. 1988 Nov 4;55(3):395-7 PMID: 3141060
  12. GAL4-VP16 is an unusually potent transcriptional activator.
    Nature. 1988 Oct 6;335(6190):563-4 PMID: 3047590
  13. A novel genetic system to detect protein-protein interactions.
    Nature. 1989 Jul 20;340(6230):245-6 PMID: 2547163
  14. A vector for expressing GAL4(1-147) fusions in mammalian cells.
    Nucleic Acids Res. 1989 Sep 25;17(18):7539 PMID: 2798115
  15. An LFA-3 cDNA encodes a phospholipid-linked membrane protein homologous to its receptor CD2.
    Nature. 1987 Oct 29-Nov 4;329(6142):840-2 PMID: 3313052
  16. A novel expression assay to study transcriptional activators.
    Gene. 1990 May 14;89(2):211-21 PMID: 2165020
  17. Searching for peptide ligands with an epitope library.
    Science. 1990 Jul 27;249(4967):386-90 PMID: 1696028
  18. Activators and targets.
    Nature. 1990 Jul 26;346(6282):329-31 PMID: 2142753
  19. A specific member of the ATF transcription factor family can mediate transcription activation by the adenovirus E1a protein.
    Cell. 1990 Jun 29;61(7):1217-24 PMID: 2142019
  20. Direct cloning of leucine zipper proteins: Jun binds cooperatively to the CRE with CRE-BP1.
    Oncogene. 1990 Apr;5(4):451-8 PMID: 2139203
  21. Both the basic region and the 'leucine zipper' domain of the cyclic AMP response element binding (CREB) protein are essential for transcriptional activation.
    EMBO J. 1990 Jan;9(1):225-32 PMID: 2136830
  22. pSH4: a mammalian cDNA expression vector.
    Nucleic Acids Res. 1990 Jun 25;18(12):3668 PMID: 2362830
  23. Isolation of mutants of an animal virus in bacteria.
    Science. 1980 Sep 19;209(4463):1392-6 PMID: 6251547
  24. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  25. Selective extraction of polyoma DNA from infected mouse cell cultures.
    J Mol Biol. 1967 Jun 14;26(2):365-9 PMID: 4291934
  26. Construction of a uniform-abundance (normalized) cDNA library.
    Proc Natl Acad Sci U S A. 1991 Mar 1;88(5):1943-7 PMID: 1705712
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.23.10686
Published
1991-12-01
Pages
10686-90
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC52995
Subset
IM
Grants
NCI NIH HHS · R35 CA42556-06 · United States
External Links
PubMed source DOI: 10.1073/pnas.88.23.10686 PMC Full Text View journal details
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