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PMID: 1661734 Published · ppublish English Journal Article

A cell culture model of the blood-brain barrier.

The Journal of cell biology ·Vol. 115 ·No. 6 ·1991-12-00 ·Pages 1725-35

Rubin LL, Hall DE, Porter S, Barbu K, Cannon C, Horner HC, Janatpour M, Liaw CW, Manning K, Morales J

Abstract

Endothelial cells that make up brain capillaries and constitute the blood-brain barrier become different from peripheral endothelial cells in response to inductive factors found in the nervous system. We have established a cell culture model of the blood-brain barrier by treating brain endothelial cells with a combination of astrocyte-conditioned medium and agents that elevate intracellular cAMP. These cells form high resistance tight junctions and exhibit low rates of paracellular leakage and fluid-phase endocytosis. They also undergo a dramatic structural reorganization as they form tight junctions. Results from these studies suggest modes of manipulating the permeability of the blood-brain barrier, potentially providing the basis for increasing the penetration of drugs into the central nervous system.

MeSH Terms
Animals Astrocytes/cytology,metabolism Biological Transport Blood-Brain Barrier Cattle Cells, Cultured Clone Cells Culture Media Cyclic AMP/metabolism Cyclic GMP/metabolism Endocytosis Endothelium/cytology,metabolism Humans Immunohistochemistry Intercellular Junctions/metabolism Kinetics Models, Biological Rats
Chemicals
Culture Media Cyclic AMP Cyclic GMP
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Rubin L L
Athena Neurosciences, Inc., South San Francisco, California 94080.
Hall D E
Porter S
Barbu K
Cannon C
Horner H C
Janatpour M
Liaw C W
Manning K
Morales J
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.115.6.1725
Published
1991-12-00
Pages
1725-35
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2289219
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.115.6.1725 PMC Full Text View journal details
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