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PMID: 16625152 Published · ppublish English Journal Article Review

Visualization of molecular interactions by fluorescence complementation.

Nature reviews. Molecular cell biology ·Vol. 7 ·No. 6 ·2006-06-00 ·Pages 449-56

Kerppola TK

Abstract

The visualization of protein complexes in living cells enables the examination of protein interactions in their normal environment and the determination of their subcellular localization. The bimolecular fluorescence complementation assay has been used to visualize interactions among multiple proteins in many cell types and organisms. Modified forms of this assay have been used to visualize the competition between alternative interaction partners and the covalent modification of proteins by ubiquitin-family peptides.

MeSH Terms
Microscopy, Fluorescence Protein Interaction Mapping/methods Proteins/metabolism Spectrometry, Fluorescence Ubiquitin/metabolism
Chemicals
Proteins Ubiquitin
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Kerppola Tom K
Howard Hughes Medical Institute and Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, Michigan 48109-0650, USA. [email protected]
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Article Info
Journal
Nature reviews. Molecular cell biology
Abbr.
Nat Rev Mol Cell Biol
ISSN
1471-0072
DOI
10.1038/nrm1929
Email
[email protected]
Published
2006-06-00
Pages
449-56
Language
English
Region
England
NLM ID
100962782
PMCID
PMC2512262
Subset
IM
Grants
Howard Hughes Medical Institute · United States
External Links
PubMed source DOI: 10.1038/nrm1929 PMC Full Text View journal details
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