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PMID: 1665124 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Preferential Y chromosomal location of TRIM, a novel transposable element of Drosophila miranda, obscura group.

Chromosoma ·Vol. 101 ·No. 3 ·1991-12-00 ·Pages 169-79

Steinemann M, Steinemann S

Abstract

We have isolated a novel transposable element from the Y chromosome of Drosophila miranda (obscura group) which shows an organization intermediate between that of typical retroviruses and the I factor of Drosophila melanogaster. The site of integration of this element, TRIM, is an inverted repeat. On the 5' side the central region of TRIM is bordered by a sequence homologous to the tRNA primer binding site (PBS) and on the 3' side by a sequence homologous to the polypurine tract (PPT). The 5' LTR (long terminal repeat) contains transcription signals, such as a TATA box and a polyadenylation sequence. Three long open reading frames (ORFs) are found within the central region of the transposon. ORF1 and ORF2 show amino acid homology to the I factor of D. melanogaster and to conserved amino acid residues of retroviral reverse transcriptases. When used as a probe for in situ hybridization the TRIM element labels about 25 euchromatic sites and the chromocenter in polytene nuclei of D. miranda females. In the chromosome complement of males the polytenized part of the Y chromosome shows about the same number of additional TRIM homologous elements. Thus, as a result of this enrichment of TRIM elements in the Y chromosome, the genomic size of the TRIM population in D. miranda males is approximately doubled.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Blotting, Southern Chromosome Mapping DNA DNA Transposable Elements Drosophila/genetics Female Gene Expression Regulation Male Molecular Sequence Data Nucleic Acid Hybridization Open Reading Frames Proteins/chemistry,genetics Restriction Mapping Retroviridae/genetics Sequence Homology, Nucleic Acid Y Chromosome
Chemicals
DNA Transposable Elements Proteins DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Steinemann M
Institut für Genetik und Mikrobiologie, Universität München, Federal Republic of Germany.
Steinemann S
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Article Info
Journal
Chromosoma
Abbr.
Chromosoma
ISSN
0009-5915
DOI
10.1007/BF00355366
Published
1991-12-00
Pages
169-79
Language
English
Region
Austria
NLM ID
2985138R
Subset
IM
Databases
GENBANK
S60911, S60914, S60917, S60919, S60920, S60969, S86344, X55038, X55039, X59239
External Links
PubMed source DOI: 10.1007/BF00355366 View journal details
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