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PMID: 16663896 Published · ppublish English Journal Article

Methylation Pattern of Radish (Raphanus sativus) Nuclear Ribosomal RNA Genes.

Plant physiology ·Vol. 76 ·No. 3 ·1984-11-00 ·Pages 627-32

Delseny M, Laroche M, Penon P

Abstract

The methylation pattern of radish Raphanus sativus nuclear rDNA has been investigated using the Hpa II, Msp I, and Hha I restriction enzymes. The presence of numerous target sites for these enzymes has been shown using cloned rDNA fragments. A large fraction of the numerous rDNA units are heavily methylated, being completely resistant to Hpa II and Hpa I. However, specific sites are constantly available in another fraction of the units and are therefore unmethylated. The use of different probes allowed us to demonstrate that hypomethylated sites are present in different regions. Major hypomethylated Hha I sites have been mapped in the 5' portion of 25S rRNA coding sequence. Among the hypomethylated fraction, different methylation patterns coexist. It has been possible to demonstrate that methylation patterns are specific for particular units. The Hha I pattern of rDNA in tissues of different developmental stages was analyzed. Evidence for possible tissue specific differences in the methylation pattern is reported.

Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Delseny M
Laboratoire de Physiologie Végétale, E.R.A. No. 226 du CNRS, Universite de Perpignan, 66025 Perpignan-Cedex, France.
Laroche M
Penon P
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.76.3.627
Published
1984-11-00
Pages
627-32
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC1064345
External Links
PubMed source DOI: 10.1104/pp.76.3.627 PMC Full Text View journal details
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