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PMID: 16666637 Published · ppublish English Journal Article

Phosphatidylinositol(4,5)bisphosphate and Phosphatidylinositol(4)phosphate in Plant Tissues.

Plant physiology ·Vol. 89 ·No. 3 ·1989-03-00 ·Pages 888-92

Irvine RF, Letcher AJ, Lander DJ, Drøbak BK, Dawson AP, Musgrave A

Abstract

Pea (Pisum sativum) leaf discs or swimming suspensions of Chlamydomonas eugametos were radiolabeled with [(3)H]myo-inositol or [(32)P]Pi and the lipids were extracted, deacylated, and their glycerol moieties removed. The resulting inositol trisphosphate and bisphosphate fractions were examined by periodate degradation, reduction and dephosphorylation, or by incubation with human red cell membranes. Their likely structures were identified as d-myo-inositol(1,4,5)trisphosphate and d-myo-inositol(1,4,)-bisphosphate. It is concluded that plants contain phosphatidylinositol(4)phosphate and phosphatidylinositol(4,5)bisphosphate; no other polyphosphoinositides were detected.

Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Irvine R F
Department of Biochemistry, Institute of Animal Physiology & Genetics Research, Babraham, Cambridge CB2 4AT, United Kingdom.
Letcher A J
Lander D J
Drøbak B K
Dawson A P
Musgrave A
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.89.3.888
Published
1989-03-00
Pages
888-92
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC1055939
External Links
PubMed source DOI: 10.1104/pp.89.3.888 PMC Full Text View journal details
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