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PMID: 16667878 Published · ppublish English Journal Article

Stable Accumulation of Modified 2S Albumin Seed Storage Proteins with Higher Methionine Contents in Transgenic Plants.

Plant physiology ·Vol. 94 ·No. 3 ·1990-11-00 ·Pages 970-9

De Clercq A, Vandewiele M, Van Damme J, Guerche P, Van Montagu M, Vandekerckhove J, Krebbers E

Abstract

We present the results of two sets of experiments designed to express high methionine proteins in transgenic seeds in three different plant species. In the first approach, two chimeric genes were constructed in which parts of the Arabidopsis 2S albumin gene 1 (AT2S1) were fused at different positions to a Brazil nut 2S albumin cDNA clone. Brazil nut 2S albumin was found to accumulate stably in transgenic Arabidopsis, Brassica napus, and tobacco seeds. In the second approach, methionine-enriched AT2S1 genes were constructed by deleting sequences encoding a region of the protein which is not highly conserved among 2S albumins of different species and replacing them with methioninerich sequences. Introduction of the modified AT2S1 genes into three different plant species resulted in the accumulation of the methionine-enriched 2S albumins in all three species at levels reaching 1 to 2% of the total high salt-extractable seed protein.

Authors & Affiliations
7 authors, click to expand affiliations / ORCID
De Clercq A
Laboratorium voor Genetica, Rijksuniversiteit Gent, B-9000 Gent, Belgium.
Vandewiele M
Van Damme J
Guerche P
Van Montagu M
Vandekerckhove J
Krebbers E
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.94.3.970
Published
1990-11-00
Pages
970-9
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC1077329
External Links
PubMed source DOI: 10.1104/pp.94.3.970 PMC Full Text View journal details
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