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PMID: 2563150 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nuclear transcriptional activity of the tobacco plastid psbA promoter.

Nucleic acids research ·Vol. 17 ·No. 1 ·1989-01-11 ·Pages 19-29

Cornelissen M, Vandewiele M

Abstract

The plastid psbA promoter of tobacco was used with the aim to construct plastid specific marker genes. Upon transfer to the tobacco nuclear genome the plastid promoter fragment appeared to specify a messenger RNA. Placed 5' to the bar or nptII coding sequences the level of expression is sufficient to obtain a selectable phenotype. The transcription start site in the nucleus is site specific and is located 4 nucleotides downstream relative to the start site used in plastids. Translational fusions of the psbA coding sequence with the nptII and bar coding sequences revealed that the psbA leader sequence and the psbA translation start codon, being the second ATG codon, are recognized by the plant cytoplasmic translation apparatus. A promoter cassette utilisable in both E. coli and tobacco, was obtained by placing the CaMV 35S enhancer 5' to the psbA promoter.

MeSH Terms
Base Sequence Cell Nucleus/metabolism Genes, Homeobox Genetic Vectors Molecular Sequence Data Plants/genetics Plants, Toxic Plasmids Promoter Regions, Genetic RNA, Messenger/genetics Restriction Mapping Tobacco/genetics Transcription, Genetic
Chemicals
RNA, Messenger
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Cornelissen M
Plant Genetic Systems NV, Gent, Belgium.
Vandewiele M
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/17.1.19
Published
1989-01-11
Pages
19-29
Language
English
Region
England
NLM ID
0411011
PMCID
PMC331532
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/17.1.19 PMC Full Text View journal details
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