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PMID: 16699543 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Arabidopsis thaliana histone deacetylase 1 (AtHD1) is localized in euchromatic regions and demonstrates histone deacetylase activity in vitro.

Cell research ·Vol. 16 ·No. 5 ·2006-05-00 ·Pages 479-88

Fong PM, Tian L, Chen ZJ

Abstract

Arabidopsis thaliana histone deacetylase 1 (AtHD1 or AtHDA19), a homolog of yeast RPD3, is a global regulator of many physiological and developmental processes in plants. In spite of the genetic evidence for a role of AtHD1 in plant gene regulation and development, the biochemical and cellular properties of AtHD1 are poorly understood. Here we report cellular localization patterns of AtHD1 in vivo and histone deacetylase activity in vitro. The transient and stable expression of a green fluorescent protein (GFP)-tagged AtHD1 in onion cells and in roots, seeds and leaves of the transgenic Arabidopsis, respectively, revealed that AtHD1 is localized in the nucleus presumably in the euchromatic regions and excluded from the nucleolus. The localization patterns of AtHD1 are different from those of AtHD2 and AtHDA6 that are involved in nucleolus formation and silencing of transgenes and repeated DNA elements, respectively. In addition, a histone deacetylase activity assay showed that the recombinant AtHD1 produced in bacteria demonstrated a specific histone deacetylase activity in vitro. The data suggest that AtHD1 is a nuclear protein and possesses histone deacetylase activities responsible for global transcriptional regulation important to plant growth and development.

MeSH Terms
Acetylation Arabidopsis/enzymology,genetics,growth & development Arabidopsis Proteins/genetics,metabolism Gene Expression Regulation, Developmental Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant Green Fluorescent Proteins/genetics,metabolism Histone Deacetylases/genetics,metabolism Plant Proteins/genetics,metabolism Plant Roots/enzymology,genetics,growth & development Plants, Genetically Modified/enzymology,genetics,growth & development Seeds/enzymology,genetics,growth & development
Chemicals
Arabidopsis Proteins Plant Proteins Green Fluorescent Proteins Histone Deacetylases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Fong Paulus M
Department of Soil and Crop Sciences, Texas A&M University, College Station, TX 77843, USA.
Tian Lu
Chen Z Jeffrey
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Article Info
Journal
Cell research
Abbr.
Cell Res
ISSN
1001-0602
DOI
10.1038/sj.cr.7310059
Published
2006-05-00
Pages
479-88
Language
English
Region
England
NLM ID
9425763
PMCID
PMC1986662
Subset
IM
Grants
NIGMS NIH HHS · R01 GM067015 · United States
NIGMS NIH HHS · R01 GM067015-03 · United States
NIGMS NIH HHS · GM067015 · United States
External Links
PubMed source DOI: 10.1038/sj.cr.7310059 PMC Full Text View journal details
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