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PMID: 1672737 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cell-type specific activity of two glucocorticoid responsive units of rat tyrosine aminotransferase gene is associated with multiple binding sites for C/EBP and a novel liver-specific nuclear factor.

Nucleic acids research ·Vol. 19 ·No. 1 ·1991-01-11 ·Pages 131-9

Grange T, Roux J, Rigaud G, Pictet R

Abstract

The structures of two remote glucocorticoid responsive units (GRUs) that cooperatively interact to promote cell-type specific glucocorticoid induction of rat tyrosine aminotransferase gene expression have been analyzed. DNAase I footprinting and gel mobility shift analyses reveal a complex array of contiguous and overlapping sites for cell type-specific DNA binding proteins. Apart from the glucocorticoid receptor, two liver-specific nuclear factors possess multiple binding sites in each of these GRUs: C/EBP and a newly identified liver-specific factor: HNF5. C/EBP possesses four binding sites in each GRU; a DNA-binding protein with similar binding specificity has been identified in fibroblasts; this protein could be related to AP-3. HNF5 possesses two binding sites in one GRU and four in the other. There are also HNF5 binding sites in numerous regulatory regions of other liver-specific genes. The interaction of HNF5 with DNA gives a characteristic DNAase I footprint with hypersensitive sites in the middle of the recognition sequence. Some of the C/EBP and HNF5 binding sites overlap in a conserved arrangement.

MeSH Terms
Animals Base Sequence Binding Sites Binding, Competitive CCAAT-Enhancer-Binding Proteins DNA DNA-Binding Proteins/metabolism Deoxyribonuclease I/metabolism Glucocorticoids/metabolism Liver/metabolism Molecular Sequence Data Nuclear Proteins/metabolism Organ Specificity Rats Transcription Factors/metabolism Tyrosine Transaminase/genetics,metabolism
Chemicals
CCAAT-Enhancer-Binding Proteins DNA-Binding Proteins Glucocorticoids Nuclear Proteins Transcription Factors DNA Tyrosine Transaminase Deoxyribonuclease I
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Grange T
Institut Jacques Monod du CNRS, Université Paris, France.
Roux J
Rigaud G
Pictet R
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/19.1.131
Published
1991-01-11
Pages
131-9
Language
English
Region
England
NLM ID
0411011
PMCID
PMC333543
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/19.1.131 PMC Full Text View journal details
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