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PMID: 16780600 Published · epublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Comparative genomics and evolution of the HSP90 family of genes across all kingdoms of organisms.

BMC genomics ·Vol. 7 ·2006-06-17 ·Pages 156

Chen B, Zhong D, Monteiro A

Abstract

HSP90 proteins are essential molecular chaperones involved in signal transduction, cell cycle control, stress management, and folding, degradation, and transport of proteins. HSP90 proteins have been found in a variety of organisms suggesting that they are ancient and conserved. In this study we investigate the nuclear genomes of 32 species across all kingdoms of organisms, and all sequences available in GenBank, and address the diversity, evolution, gene structure, conservation and nomenclature of the HSP90 family of genes across all organisms. Twelve new genes and a new type HSP90C2 were identified. The chromosomal location, exon splicing, and prediction of whether they are functional copies were documented, as well as the amino acid length and molecular mass of their polypeptides. The conserved regions across all protein sequences, and signature sequences in each subfamily were determined, and a standardized nomenclature system for this gene family is presented. The proeukaryote HSP90 homologue, HTPG, exists in most Bacteria species but not in Archaea, and it evolved into three lineages (Groups A, B and C) via two gene duplication events. None of the organellar-localized HSP90s were derived from endosymbionts of early eukaryotes. Mitochondrial TRAP and endoplasmic reticulum HSP90B separately originated from the ancestors of HTPG Group A in Firmicutes-like organisms very early in the formation of the eukaryotic cell. TRAP is monophyletic and present in all Animalia and some Protista species, while HSP90B is paraphyletic and present in all eukaryotes with the exception of some Fungi species, which appear to have lost it. Both HSP90C (chloroplast HSP90C1 and location-undetermined SP90C2) and cytosolic HSP90A are monophyletic, and originated from HSP90B by independent gene duplications. HSP90C exists only in Plantae, and was duplicated into HSP90C1 and HSP90C2 isoforms in higher plants. HSP90A occurs across all eukaryotes, and duplicated into HSP90AA and HSP90AB in vertebrates. Diplomonadida was identified as the most basal organism in the eukaryote lineage. The present study presents the first comparative genomic study and evolutionary analysis of the HSP90 family of genes across all kingdoms of organisms. HSP90 family members underwent multiple duplications and also subsequent losses during their evolution. This study established an overall framework of information for the family of genes, which may facilitate and stimulate the study of this gene family across all organisms.

MeSH Terms
Alternative Splicing Amino Acid Sequence Animals Archaeal Proteins/chemistry,genetics Bacterial Proteins/chemistry,genetics Chromosome Mapping Complementarity Determining Regions Conserved Sequence Evolution, Molecular Genetic Variation Genomics HSP90 Heat-Shock Proteins/chemistry,genetics,physiology Molecular Sequence Data Multigene Family Sequence Alignment Sequence Homology, Amino Acid Terminology as Topic Vertebrates
Chemicals
Archaeal Proteins Bacterial Proteins Complementarity Determining Regions HSP90 Heat-Shock Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Chen Bin
School of Life Sciences, Southwest University, Chongqing 400715, P.R. China. [email protected]
Zhong Daibin
Monteiro Antónia
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Article Info
Journal
BMC genomics
Abbr.
BMC Genomics
ISSN
1471-2164
DOI
10.1186/1471-2164-7-156
Email
[email protected]
Published
2006-06-17
Epub
2006-00-17
Pages
156
Language
English
Region
England
NLM ID
100965258
PMCID
PMC1525184
Subset
IM
External Links
PubMed source DOI: 10.1186/1471-2164-7-156 PMC Full Text View journal details
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