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PMID: 16789150 Published · ppublish English Journal Article

Chromatographic Separation and Antigenic Analysis of Proteins of the Oncornaviruses IV. Biochemical Typing of Murine Viral Proteins.

Journal of virology ·Vol. 15 ·No. 5 ·1975-05-00 ·Pages 1148-57

Buchhagen DL, Stutman O, Fleissner E

Abstract

Tryptic peptide maps were prepared for four purified structural proteins derived from several murine leukemia viruses (MuLV's). Analyses of these peptide maps reveal that the p30 proteins of Rauscher, Moloney, and Gross MuLV's are very similar to each other, as are the p10's obtained from these three viruses. In contrast, the peptide maps of the individual p15's and p12's from the same viruses establish that each of these polypeptides is highly strain specific. For all four polypeptides studied, unique peptides appear in the Rauscher MuLV and Moloney MuLV tryptic profiles that are not present in the corresponding Gross MuLV profile. By this method of analysis it was possible to distinguish the p30's of N-tropic and B-tropic MuLV's derived from the same BALB/c mouse.

Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Buchhagen D L
Laboratories of Viral Oncology and Cellular Immunobiology, Memorial Sloan-Kettering Cancer Center, New York, New York 10021.
Stutman O
Fleissner E
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.15.5.1148-1157.1975
Published
1975-05-00
Pages
1148-57
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC354569
External Links
PubMed source DOI: 10.1128/JVI.15.5.1148-1157.1975 PMC Full Text View journal details
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