In vitro synthesis of turnip yellow mosaic virus coat protein in a wheat germ cell-free system.

Journal of virology ·Vol. 20 ·No. 1 ·1976-10-00 ·Pages 196-202

Benicourt C, Haenni AL

Abstract

Turnip Yellow Mosaic Virus RNA directs the synthesis in vitro of its coat protein in a wheat germ cell-free extract. Optimum conditions for synthesis have been defined, and the effect of spermine on specifically enhancing coat protein formation has been examined. Identity between the in vitro synthesized coat protein and authentic coat protein of Turnip Yellow Mosaic Virus was established by analysis on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, peptide mapping, and immunoprecipitation.

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Benicourt C

Laboratoire de Biochimie du Développement, Institut de Biologie Moléculaire du Centre National de la Recherche Scientifique, Université Paris VII, 75005 Paris, France.

Haenni A L

References (28)

28 references, click to expand

Translation of the RNAs of brome mosaic virus: the monocistronic nature of RNA1 and RNA2.
J Mol Biol. 1976 May 5;103(1):77-88 PMID: 957426
Messenger RNA for the coat protein of tobacco mosaic virus.
Nature. 1976 Apr 29;260(5554):759-64 PMID: 1264250
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
Binding and translation of turnip-yellow-mosaic-virus ribonucleic acid by skeletal-muscle ribosomes from normal and diabetic rats.
Biochem J. 1970 May;117(5):899-905 PMID: 5451911
Tobacco mosaic virus ribonucleic acid-dependent amino acid incorporation in a wheat embryo system in vitro. Analysis of the rate-limiting reaction.
J Biol Chem. 1970 Mar 10;245(5):955-61 PMID: 5417266
The isolation of plant viruses by means of "simple" coacervates.
Virology. 1966 Nov;30(3):341-7 PMID: 5921640
Binding of transfer RNA to polyamines in preference to Mg-2+.
Biochem Biophys Res Commun. 1975 Apr 7;63(3):611-7 PMID: 1093544
Polyamines in bacteriophage R17 and its RNA.
J Virol. 1975 Aug;16(2):222-7 PMID: 1097731
The determination of amino acids by high-voltage paper electrophoresis.
Methods Biochem Anal. 1965;13:1-45 PMID: 5324936
Bacteriophage T4 DNA-dependent in vitro synthesis of lysozyme.
Proc Natl Acad Sci U S A. 1969 Aug;63(4):1351-8 PMID: 4901474
Translation of TMV-RNA in a cell-free wheat embryo system.
Virology. 1973 Jun;53(2):343-8 PMID: 4712387
Fractionation of cucumber mosaic virus RNA and its translation in a wheat embryo cell-free system.
Virology. 1975 Jan;63(1):252-62 PMID: 163049
Translation of brome mosaic viral ribonucleic acid in a cell-free system derived from wheat embryo.
Proc Natl Acad Sci U S A. 1973 Jun;70(6):1799-803 PMID: 4515941
Translation of satellite tobacco necrosis virus ribonucleic acid. I. Characterization of in vitro procaryotic and eucaryotic translation products.
Biochemistry. 1972 May 23;11(11):2009-14 PMID: 4554894
Translation of virus mRNA: synthesis of bacteriophage Q beta proteins in a cell-free extract from wheat embryo.
J Virol. 1973 Dec;12(6):1434-41 PMID: 4586778
Bacteriophage Q replicase contains the protein biosynthesis elongation factors EF Tu and EF Ts.
Proc Natl Acad Sci U S A. 1972 May;69(5):1313-7 PMID: 4624757
Simian virus 40 DNA directs synthesis of authentic viral polypeptides in a linked transcription-translation cell-free system.
Proc Natl Acad Sci U S A. 1975 May;72(5):1922-6 PMID: 168582
Synthesis of polyoma proteins in vitro.
J Mol Biol. 1973 Mar 15;74(4):627-34 PMID: 4354076
Virus protein synthesis in animal cell-free systems: nature of the products synthesized in resonse to ribonucleic acid of encephalomyocarditis virus.
J Virol. 1971 Apr;7(4):438-47 PMID: 4329561
Primary structure of turnip yellow mosaic virus coat protein.
Virology. 1972 Aug;49(2):615-7 PMID: 4340812
Translation of virus mRNA: protein synthesis directed by several virus RNAs in a cell-free extract from wheat germ.
J Gen Virol. 1974 Oct;25(1):11-20 PMID: 4372305
Elongation factor-viral genome interaction dependent on the aminoacylation of TYMV and TMV RNAs.
Nat New Biol. 1973 Jan 17;241(107):88-90 PMID: 4572945
Reconstitution of Q replicase lacking subunit with protein-synthesis-interference factor i.
Eur J Biochem. 1972 Nov 21;31(1):44-51 PMID: 4640466
Characterization of a highly efficient protein synthesizing system derived from commercial wheat germ.
Nucleic Acids Res. 1974 Nov;1(11):1385-97 PMID: 10793698
Degradation of tobacco mosaic virus with acetic acid.
Virology. 1957 Aug;4(1):1-4 PMID: 13456355
THE READ-OUT OF TURNIP YELLOW MOSAIC VIRUS RIBONUCLEIC ACID AS A POLYCISTRONIC MESSENGER IN CELL-FREE SYSTEMS OF ESCHERICHIA COLI.
Biochim Biophys Acta. 1965 Mar 15;95:446-60 PMID: 14342537
Infectivity of ribonucleic acid from tobacco mosaic virus.
Nature. 1956 Apr 14;177(4511):702-3 PMID: 13321939
Efficient translation of tobacco mosaic virus RNA and rabbit globin 9S RNA in a cell-free system from commercial wheat germ.
Proc Natl Acad Sci U S A. 1973 Aug;70(8):2330-4 PMID: 4525168

Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.20.1.196-202.1976

Published

1976-10-00

Pages

196-202

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC354980

External Links

PubMed source DOI: 10.1128/JVI.20.1.196-202.1976 PMC Full Text View journal details

Analysis Services

In vitro synthesis of turnip yellow mosaic virus coat protein in a wheat germ cell-free system.

Contact

WeChat Official Account